Rabbit Polyclonal EPAS-1 antibody (STJ96690)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal EPAS-1 antibody (STJ96690)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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EPAS1 antibody, BHLHE73 antibody, HIF2A antibody, MOP2 antibody, PASD2 antibody,|Basic helix loop helix PAS protein MOP2 antibody|Basic-helix-loop-helix-PAS protein MOP2 antibody|bHLHe73 antibody|Class E basic helix-loop-helix protein 73 antibody|ECYT4 antibody|Endothelial PAS domain containing protein 1 antibody|Endothelial pas domain protein 1 antibody|Endothelial PAS domain-containing protein 1 antibody|EPAS 1 antibody|EPAS-1 antibody|EPAS1 antibody|EPAS1_HUMAN antibody|HIF 1 alpha like factor antibody|HIF 2 alpha antibody|HIF-1-alpha-like factor antibody|HIF-2-alpha antibody|HIF2-alpha antibody|HIF2A antibody|HLF antibody|Hypoxia inducible factor 2 alpha antibody|Hypoxia inducible factor 2 alpha subunit antibody|Hypoxia-inducible factor 2-alpha antibody|Member of PAS protein 2 antibody|Member of pas superfamily 2 antibody|MOP 2 antibody|MOP2 antibody|PAS domain-containing protein 2 antibody|PASD2 antibody|Anti-HIF-2-alpha antibody – ChIP Grade (ab199)
SCBT cat No: sc-13596|sc-46691|sc-8712|sc-28706|sc-32146|

Name

EPAS-1 Polyclonal Antibody

Catalogue No.

STJ96690

Reactivity

Human, Mouse, Rat

Specificity

EPAS-1 Polyclonal Antibody detects endogenous levels of EPAS-1 protein.

Immunogen

Synthesized peptide derived from human EPAS-1 around the non-acetylation site of K385

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

JK/50/96/120, L929/50/96/30

Formulation

EPAS-1 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

EPAS-1 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Endothelial PAS domain-containing protein 1 antibody, EPAS-1 antibody, Basic-helix-loop-helix-PAS protein MOP2 antibody, Class E basic helix-loop-helix protein 73 antibody, bHLHe73 antibody, HIF-1-alpha-like factor antibody, HLF antibody, Hypoxia-inducible factor 2-alpha antibody, HIF-2-alpha antibody, HIF2-alpha antibody, Member of PAS protein 2 antibody, PAS domain-containing protein 2 antibody

Database links

Human UniProt/Swiss-Prot:Q99814;Mouse UniPort/Swiss-Prot: P97481;Rat UniProt/Swiss-Port: Q9JHS1;Human Entrez Gene: 2034;Mouse Entrez Gene: 13819;Rat Entrez Gene: Rn.163791

Protein names

Endothelial PAS domain-containing protein 1 , EPAS-1 , Basic-helix-loop-helix-PAS protein MOP2 , Class E basic helix-loop-helix protein 73 , bHLHe73 , HIF-1-alpha-like factor , HLF , Hypoxia-inducible factor 2-alpha , HIF-2-alpha , HIF2-alpha , Member of PAS protein 2 , PAS domain-containing protein 2

Protein function

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5′-[AG]CGTG-3′ within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Protein tissue specificity

Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Involvement in disease

Erythrocytosis, familial, 4 (ECYT4) [MIM:611783]: An autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal platelet and leukocyte counts. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Contains 1 bHLH (basic helix-loop-helix) domain. / Contains 1 PAC (PAS-associated C-terminal) domain. / Contains 2 PAS (PER-ARNT-SIM) domains.

Protein post-translational modifications

In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity). / In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. / Phosphorylated on multiple sites in the CTAD. / The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

Protein cellular localization

Nucleus / Nucleus speckle

Research area

All research areas>Transcription Regulators>EPAS
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize EPAS-1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human stomach tissue

1: EPAS-1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach tissue

1: EPAS-1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: EPAS-1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: EPAS-1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: EPAS-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat heart tissue

1: EPAS-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: EPAS-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse heart tissue

1: EPAS-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: EPAS-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: EPAS-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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