Rabbit Polyclonal ERbeta antibody (STJ93002)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Antibody Validation Project, Antibody Trial Sample, Antibody Free Sample for your own antibody validation and submitting antibody reviews, AntibodyPlus

Rabbit Polyclonal ERbeta antibody (STJ93002)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for ERbeta Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

ESR2 antibody, ESTRB antibody, NR3A2 antibody,|Atherosclerosis, susceptibility to, included antibody|DKFZp686N23123 antibody|ER Alpha antibody|ER antibody|ER Beta antibody|ER-alpha antibody|ER[a] antibody|ER[b] antibody|Era antibody|ERalpha antibody|Erb antibody|Erb2 antibody|ERbeta antibody|ESR antibody|ESR BETA antibody|ESR1 antibody|ESR1_HUMAN antibody|ESR2 antibody|ESRA antibody|ESRB antibody|Estr antibody|Estra antibody|Estradiol Receptor alpha antibody|Estradiol receptor antibody|Estradiol Receptor beta antibody|ESTRB antibody|Estrogen receptor 1 (alpha) antibody|Estrogen Receptor 1 antibody|Estrogen receptor 2 (ER beta) antibody|Estrogen Receptor 2 antibody|Estrogen receptor 2 ER beta antibody|Estrogen receptor alpha antibody|Estrogen receptor antibody|Estrogen receptor beta 4 antibody|Estrogen resistance, included antibody|HDL cholesterol, augmented response of, to hormone replacement, included antibody|Myocardial infarction, susceptibility to, included antibody|NR3A1 antibody|NR3A2 antibody|Nuclear receptor subfamily 3 group A member 1 antibody|Nuclear receptor subfamily 3 group A member 2 antibody|OTTHUMP00000017718 antibody|OTTHUMP00000017719 antibody|RNESTROR antibody|Anti-Estrogen Receptor antibody (ab53572)
SCBT cat No: sc-53494|sc-390243|sc-373853|sc-8974|sc-6822|sc-6820|sc-6821|

Name

ERbeta Polyclonal Antibody

Catalogue No.

STJ93002

Reactivity

Human, Mouse, Rat

Specificity

ERbeta Polyclonal Antibody detects endogenous levels of ERbeta protein.

Immunogen

Synthesized peptide derived from ERbeta at AA range 40-120

Host

Rabbit

Applications

WB, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

59 kDa

Formulation

ERbeta Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

ERbeta Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Estrogen receptor beta antibody, ER-beta antibody, Nuclear receptor subfamily 3 group A member 2 antibody

Database links

Human UniProt/Swiss-Prot:Q92731;Mouse UniPort/Swiss-Prot: O08537;Rat UniProt/Swiss-Port: Q62986;Human Entrez Gene: 2100;Mouse Entrez Gene: 13983;Rat Entrez Gene: Rn.37460

Protein names

Estrogen receptor beta , ER-beta , Nuclear receptor subfamily 3 group A member 2

Protein function

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner . Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

Protein tissue specificity

Isoform 1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform 2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform 4 is found in testis. Isoform 5 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform 6 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

Protein sequence and domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. / Belongs to the nuclear hormone receptor family. NR3 subfamily. / Contains 1 nuclear receptor DNA-binding domain.

Protein post-translational modifications

Phosphorylation at Ser-87 and Ser-105 recruits NCOA1.

Protein cellular localization

Nucleus

Research area

All research areas>Steroid Receptors>ER beta
(View all antibody categories related to Steroid Receptors)

Note

AntibodyPlus can customize ERbeta Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews
 

Immunofluorescence analysis of Mouse kidney tissue

1: ERbeta Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: ERbeta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: ERbeta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat liver tissue

1: ERbeta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spinal cord tissue

1: ERbeta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat brain tissue

1: ERbeta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: ERbeta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: ERbeta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


Welcome to AntibodyPlus!