Rabbit polyclonal ESR1 antibody (A0296)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ESR1 antibody (A0296)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:1000 – 1:4000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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ESR1 antibody,DKFZp686N23123 antibody,ER antibody,ESR antibody,ESRA antibody,Era antibody,NR3A1 antibody, Estrogen Receptor alpha antibody
DKFZp686N23123 antibody|ER alpha antibody|ER antibody|ER-alpha antibody|Era antibody|ESR antibody|ESR1 antibody|ESR1_HUMAN antibody|ESRA antibody|Estradiol receptor antibody|Estrogen nuclear receptor alpha antibody|Estrogen receptor 1 antibody|Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody|Estrogen receptor alpha antibody|Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody|Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody|Estrogen receptor alpha delta 4 +49 isoform antibody|Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody|Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody|Estrogen receptor alpha E1 E2 1 2 antibody|Estrogen receptor alpha E1 N2 E2 1 2 antibody|Estrogen receptor antibody|ESTRR antibody|NR3A1 antibody|Nuclear receptor subfamily 3 group A member 1 antibody|Anti-Estrogen Receptor alpha antibody [E115] – ChIP Grade (ab32063)

SCBT cat No: sc-242022|sc-242026|sc-242025|sc-245215|sc-245234|sc-163801|sc-101902|sc-162584|sc-162586|sc-245256|sc-240033|sc-247666|sc-139226|sc-245304|sc-514440|sc-242049|sc-138158|sc-163818|sc-163819|sc-514159|sc-242055|sc-245364|sc-245362|sc-245365|sc-514699|sc-514707|sc-242068|sc-136736|sc-160185|sc-390887|sc-390559|sc-393065|sc-167299|sc-240057|sc-240056|sc-398999|sc-398998|sc-81862|sc-245464|sc-245465|sc-245468|sc-241243|sc-245470|sc-137978|sc-137980|sc-515388|sc-393861|sc-242108|sc-242107|sc-245492|sc-393896|

Name

Rabbit polyclonal ESR1 antibody

Catalogue No.

A0296

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human ESR1

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:1000 – 1:4000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 66kDa/Observed: Refer to Figures

Formulation

ESR1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ESR1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ESR1 antibody,DKFZp686N23123 antibody,ER antibody,ESR antibody,ESRA antibody,Era antibody,NR3A1 antibody, Estrogen Receptor alpha antibody

Database links

Human UniProt/Swiss-Prot:P03372

Protein names

ESR1,DKFZp686N23123,ER,ESR,ESRA,Era,NR3A1, Estrogen Receptor alpha

Protein function

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.

Protein tissue specificity

Widely expressed. Isoform 3 is not expressed in the pituitary gland.

Protein sequence and domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. The modulating domain, also known as A/B or AF-1 domain has a ligand-independent transactivation function. The C-terminus contains a ligand-dependent transactivation domain, also known as E/F or AF-2 domain which overlaps with the ligand binding domain. AF-1 and AF-2 activate transcription independently and synergistically and act in a promoter- and cell-specific manner. AF-1 seems to provide the major transactivation function in differentiated cells.Belongs to the nuclear hormone receptor family. NR3 subfamily.; Contains 1 nuclear receptor DNA-binding domain.

Protein post-translational modifications

Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro.; Glycosylated; contains N-acetylglucosamine, probably O-linked.; Ubiquitinated. Deubiquitinated by OTUB1.; Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.; Palmitoylated (isoform 3). Not biotinylated (isoform 3).; Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor.

Protein cellular localization

Isoform 1: Nucleus . Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Note: A minor fraction is associated with the inner membrane.; Isoform 3: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Single-pass type I membrane protein. Note: Associated with the inner membrane via palmitoylation (Probable). At least a subset exists as a transmembrane protein with a N-terminal extracellular domain.; Nucleus. Golgi apparatus. Cell membrane. Note: Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated.

Background

Estrogen receptor alpha (ERalpha), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERalpha regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ERalpha activity (3,4). ERalpha is phosphorylated on multiple sites (5). Ser104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ERalpha activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4).

Note

AntibodyPlus can customize ESR1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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