Rabbit polyclonal EYA3 antibody (A7893)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal EYA3 antibody (A7893)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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AI844637 antibody|DKFZp686C132 antibody|EYA3 antibody|EYA3_HUMAN antibody|Eyes absent 3 antibody|Eyes absent 3 homolog (Drosophila) antibody|Eyes absent homolog 3 antibody|OTTMUSP00000010370 antibody|RP11-460I13.4 antibody|RP23-442A20.1 antibody|Anti-EYA3 antibody (ab22835)

SCBT cat No: sc-15101|

Name

Rabbit polyclonal EYA3 antibody

Catalogue No.

A7893

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human EYA3

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 63kDa/Observed: Refer to figures

Formulation

EYA3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

EYA3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Database links

Human UniProt/Swiss-Prot:Q99504

Protein function

Tyrosine phosphatase that specifically dephosphorylates ‘Tyr-142’ of histone H2AX (H2AXY142ph). ‘Tyr-142’ phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 . Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.

Protein sequence and domain

Belongs to the HAD-like hydrolase superfamily. EYA family.

Protein post-translational modifications

Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.

Protein cellular localization

Cytoplasm . Nucleus . Note: Localizes at sites of DNA damage at double-strand breaks (DSBs) . With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively (By similarity).

Background

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3′ UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.

Note

AntibodyPlus can customize EYA3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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