Rabbit polyclonal FANCD2 antibody (A2072)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal FANCD2 antibody (A2072)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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DKFZp762A223 antibody, FA-D2 antibody, FA4 antibody, FACD antibody, FAD antibody, FAD2 antibody, FANCD antibody, FLJ23826 antibody
DKFZp762A223 antibody|FA 4 antibody|FA D2 antibody|FA4 antibody|FAC D2 antibody|FACD 2 antibody|FACD antibody|FACD2 antibody|FACD2_HUMAN antibody|FAD antibody|FAD2 antibody|FANC D2 antibody|FANCD 2 antibody|FANCD antibody|FANCD2 antibody|FANCONI ANEMIA COMPLEMENTATION GROUP D antibody|Fanconi anemia complementation group D2 antibody|Fanconi anemia group D2 protein antibody|FANCONI PANCYTOPENIA TYPE 4 antibody|FLJ23826 antibody|OTTHUMP00000158853 antibody|OTTHUMP00000207925 antibody|Protein FACD2 antibody|Type 4 Fanconi pancytopenia antibody|Anti-FANCD2 antibody [EPR2302] (ab108928)

SCBT cat No: sc-23584|sc-20022|sc-28194|

Name

Rabbit polyclonal FANCD2 antibody

Catalogue No.

A2072

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human FANCD2

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 166kDa/Observed: Refer to Figures

Formulation

FANCD2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

FANCD2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

DKFZp762A223 antibody, FA-D2 antibody, FA4 antibody, FACD antibody, FAD antibody, FAD2 antibody, FANCD antibody, FLJ23826 antibody

Database links

Human UniProt/Swiss-Prot:Q9BXW9

Protein names

DKFZp762A223, FA-D2, FA4, FACD, FAD, FAD2, FANCD, FLJ23826

Protein function

Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.

Protein tissue specificity

Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.

Protein sequence and domain

The C-terminal 24 residues of isoform 2 are required for its function.

Protein post-translational modifications

Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress by FANCL in complex with E2 ligases UBE2T or UBE2W (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the joint intervention of the FANC core complex, including FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, and FANCM, and proteins involved in cell cycle checkpoints and DNA repair, including RPA1, ATR, CHEK1 and BRCA1, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.; Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.

Protein cellular localization

Nucleus . Note: Concentrates in nuclear foci during S phase and upon genotoxic stress. At the onset of mitosis, excluded from chromosomes and diffuses into the cytoplasm, returning to the nucleus at the end of cell division. Observed in a few spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites and could be sites of replication fork stalling. The foci are frequently interlinked through BLM-associated ultra-fine DNA bridges. Following aphidicolin treatment, targets chromatid gaps and breaks.

Background

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Research area

All research areas>Signaling Intermediates>FANC
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize FANCD2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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