Rabbit Polyclonal FAS-L antibody (STJ93042)

$99.00$319.00

Reactivity: Human, Mouse
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal FAS-L antibody (STJ93042)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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FASLG antibody, APT1LG1 antibody, CD95L antibody, FASL antibody, TNFSF6 antibody,|ALPS1B antibody|Apoptosis (APO 1) antigen ligand 1 antibody|Apoptosis antigen ligand 1 antibody|Apoptosis antigen ligand antibody|APT1LG1 antibody|APTL antibody|CD178 antibody|CD178 antigen antibody|CD95 ligand antibody|CD95-L antibody|CD95L antibody|CD95L protein antibody|Fas antigen ligand antibody|Fas L antibody|Fas ligand (TNF superfamily member 6) antibody|Fas ligand antibody|FASL antibody|Fasl Fas ligand (TNF superfamily member 6) antibody|FASLG antibody|Generalized lymphoproliferative disease antibody|Gld antibody|soluble form antibody|TNFL6_HUMAN antibody|TNFSF6 antibody|Tumor necrosis factor (ligand) superfamily member 6 antibody|Tumor necrosis factor ligand superfamily member 6 antibody|Anti-Fas Ligand antibody (ab15285)
SCBT cat No: sc-19988|

Name

FAS-L Polyclonal Antibody

Catalogue No.

STJ93042

Reactivity

Human, Mouse

Specificity

FAS-L Polyclonal Antibody detects endogenous levels of FAS-L protein.

Immunogen

Synthesized peptide derived from FAS-L at AA range 70-150

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

33 kDa

Formulation

FAS-L Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

FAS-L Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tumor necrosis factor ligand superfamily member 6 antibody, Apoptosis antigen ligand antibody, APTL antibody, CD95 ligand antibody, CD95-L antibody, Fas antigen ligand antibody, Fas ligand antibody, FasL antibody, CD antigen CD178 antibody

Database links

Human UniProt/Swiss-Prot:P48023;Mouse UniPort/Swiss-Prot: P41047;Rat UniProt/Swiss-Port: P36940;Human Entrez Gene: 356;Mouse Entrez Gene: 14103;Rat Entrez Gene: Rn.9725

Protein names

Tumor necrosis factor ligand superfamily member 6 , Apoptosis antigen ligand , APTL , CD95 ligand , CD95-L , Fas antigen ligand , Fas ligand , FasL , CD antigen CD178

Protein function

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. / The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.

Involvement in disease

Autoimmune lymphoproliferative syndrome 1B (ALPS1B) [MIM:601859]: A disorder of apoptosis that manifests in early childhood and results in the accumulation of autoreactive lymphocytes. It is characterized by non-malignant lymphadenopathy with hepatosplenomegaly, and autoimmune hemolytic anemia, thrombocytopenia and neutropenia. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the tumor necrosis factor family.

Protein post-translational modifications

The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells. / N-glycosylated. / Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization. / Monoubiquitinated.

Protein cellular localization

Cell membrane; Single-pass type II membrane protein / Cytoplasmic vesicle lumen / Lysosome lumen / Secreted / Nucleus

Research area

All research areas>Tumor Suppressor/Apoptosis>FAS-L (CD95 Ligand)
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize FAS-L Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Rat lung tissue

1: FAS-L Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: FAS-L Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: FAS-L Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: FAS-L Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse kidney tissue

1: FAS-L Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse kidney tissue

1: FAS-L Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: FAS-L Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: FAS-L Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: FAS-L Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse colon tissue

1: FAS-L Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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