Rabbit polyclonal FasLG antibody (A0234)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal FasLG antibody (A0234)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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FASLG antibody,APT1LG1 antibody,CD178 antibody,CD95L antibody,FASL antibody,TNFSF6 antibody
ALPS1B antibody|Apoptosis (APO 1) antigen ligand 1 antibody|Apoptosis antigen ligand 1 antibody|Apoptosis antigen ligand antibody|APT1LG1 antibody|APTL antibody|CD178 antibody|CD178 antigen antibody|CD95 ligand antibody|CD95-L antibody|CD95L antibody|CD95L protein antibody|Fas antigen ligand antibody|Fas L antibody|Fas ligand (TNF superfamily member 6) antibody|Fas ligand antibody|FASL antibody|Fasl Fas ligand (TNF superfamily member 6) antibody|FASLG antibody|Generalized lymphoproliferative disease antibody|Gld antibody|soluble form antibody|TNFL6_HUMAN antibody|TNFSF6 antibody|Tumor necrosis factor (ligand) superfamily member 6 antibody|Tumor necrosis factor ligand superfamily member 6 antibody|Anti-Fas Ligand antibody (ab15285)

SCBT cat No: sc-19988|

Name

Rabbit polyclonal FasLG antibody

Catalogue No.

A0234

Reactivity

Human, Mouse

Immunogen

A synthetic peptide of human FasLG

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 31kDa/Observed: Refer to Figures

Formulation

FasLG antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

FasLG antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

FASLG antibody,APT1LG1 antibody,CD178 antibody,CD95L antibody,FASL antibody,TNFSF6 antibody

Database links

Human UniProt/Swiss-Prot:P48023

Protein names

FASLG,APT1LG1,CD178,CD95L,FASL,TNFSF6

Protein function

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.; The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.

Protein sequence and domain

Belongs to the tumor necrosis factor family.

Protein post-translational modifications

The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.; N-glycosylated.; Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.; Monoubiquitinated.

Protein cellular localization

Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen. Lysosome lumen. Note: Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane.; Tumor necrosis factor ligand superfamily member 6, soluble form: Secreted . Note: May be released into the extracellular fluid, probably by cleavage form the cell surface.; FasL intracellular domain: Nucleus. Note: The FasL ICD cytoplasmic form is translocated into the nucleus.

Background

Association of the receptor Fas with its ligand FasLG triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasLG (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4). FasLG (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-alpha, TRAIL, and TWEAK. Binding of FasLG to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasLG is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasLG is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasLG can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8,9).

Research area

All research areas>Tumor Suppressor/Apoptosis>FAS-L (CD95 Ligand)
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize FasLG antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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