Rabbit polyclonal FEN1 antibody (A1175)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal FEN1 antibody (A1175)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:10 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal FEN1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

FEN1 antibody,FEN-1 antibody,MF1 antibody,RAD2 antibody
DNase IV antibody|FEN-1 antibody|FEN1 antibody|FEN1_HUMAN antibody|Flap endonuclease 1 antibody|Flap structure specific endonuclease 1 antibody|Flap structure-specific endonuclease 1 antibody|hFEN-1 antibody|hFEN1 antibody|Maturation factor 1 antibody|MF1 antibody|Rad2 antibody|Anti-FEN1 antibody [4E7] (ab462)

SCBT cat No: sc-56675|sc-28355|sc-8795|sc-13051|sc-8794|

Name

Rabbit polyclonal FEN1 antibody

Catalogue No.

A1175

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human FEN1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:10 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 43kDa/Observed: Refer to Figures

Formulation

FEN1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

FEN1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

FEN1 antibody,FEN-1 antibody,MF1 antibody,RAD2 antibody

Database links

Human UniProt/Swiss-Prot:P39748

Protein names

FEN1,FEN-1,MF1,RAD2

Protein function

Structure-specific nuclease with 5′-flap endonuclease and 5′-3′ exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5′-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5′-end of a downstream Okazaki fragment. It enters the flap from the 5′-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5′-3′ exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Protein sequence and domain

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Protein post-translational modifications

Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.; Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.; Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.

Protein cellular localization

Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm. Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; Isoform FENMIT: Mitochondrion .

Background

Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10).

Research area

All research areas>Transcription Regulators>FEN
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize FEN1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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