Rabbit polyclonal FGFR2 antibody (A2074)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal FGFR2 antibody (A2074)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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BEK antibody, BFR-1 antibody, CD332 antibody, CEK3 antibody, CFD1 antibody, ECT1 antibody, FLJ98662 antibody, JWS antibody, K-SAM antibody, KGFR antibody, TK14 antibody, TK25 antibody
bacteria-expressed kinase antibody|BBDS antibody|BEK antibody|BEK fibroblast growth factor receptor antibody|BFR1 antibody|CD332 antibody|CD332 antigen antibody|CEK3 antibody|CFD1 antibody|Craniofacial dysostosis 1 antibody|ECT1 antibody|FGF receptor antibody|FGFR 2 antibody|FGFR-2 antibody|Fgfr2 antibody|FGFR2_HUMAN antibody|Fibroblast growth factor receptor 2 antibody|Hydroxyaryl protein kinase antibody|Jackson Weiss syndrome antibody|JWS antibody|K SAM antibody|K-sam antibody|Keratinocyte growth factor receptor 2 antibody|Keratinocyte growth factor receptor antibody|KGFR antibody|KSAM antibody|protein tyrosine kinase, receptor like 14 antibody|soluble FGFR4 variant 4 antibody|TK14 antibody|TK25 antibody|Anti-FGFR2 antibody (ab10648)

SCBT cat No: sc-122|sc-6930|sc-20735|sc-31164|

Name

Rabbit polyclonal FGFR2 antibody

Catalogue No.

A2074

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human FGFR2

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 79kDa/Observed: Refer to Figures

Formulation

FGFR2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

FGFR2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

BEK antibody, BFR-1 antibody, CD332 antibody, CEK3 antibody, CFD1 antibody, ECT1 antibody, FLJ98662 antibody, JWS antibody, K-SAM antibody, KGFR antibody, TK14 antibody, TK25 antibody

Database links

Human UniProt/Swiss-Prot:P21802

Protein names

BEK, BFR-1, CD332, CEK3, CFD1, ECT1, FLJ98662, JWS, K-SAM, KGFR, TK14, TK25

Protein function

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.

Protein sequence and domain

The second and third Ig-like domains directly interact with fibroblast growth factors (FGF) and heparan sulfate proteoglycans. Alternative splicing events affecting the third Ig-like domain are crucial for ligand selectivity.Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.; Contains 3 Ig-like C2-type (immunoglobulin-like) domains.; Contains 1 protein kinase domain.

Protein post-translational modifications

Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on several tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-769 is essential for interaction with PLCG1.; N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus.; Ubiquitinated. FGFR2 is rapidly ubiquitinated after autophosphorylation, leading to internalization and degradation. Subject to degradation both in lysosomes and by the proteasome.

Protein cellular localization

Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cytoplasmic vesicle. Note: Detected on osteoblast plasma membrane lipid rafts. After ligand binding, the activated receptor is rapidly internalized and degraded.; Isoform 1: Cell membrane; Single-pass type I membrane protein. Note: After ligand binding, the activated receptor is rapidly internalized and degraded.; Isoform 3: Cell membrane; Single-pass type I membrane protein. Note: After ligand binding, the activated receptor is rapidly internalized and degraded.; Isoform 14: Secreted.; Isoform 19: Secreted.

Background

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma (4,5).

Research area

All research areas>Membrane Receptors>Bek
(View all antibody categories related to Membrane Receptors)

Note

AntibodyPlus can customize FGFR2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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