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Rabbit polyclonal FLNA antibody (A7896)
$69.00–$259.00
Reactivity: Human,Mouse
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.
Rabbit polyclonal FLNA antibody (A7896)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC
Recommended dilution:
WB 1:500 – 1:2000 IHC 1:50 – 1:200
Recommended protocols: check protocols
Image descriptions:
Click or hover above images to see image description for Rabbit polyclonal FLNA antibody.
Alternative names:
Check alternative names for the antibody
ExpandFLN antibody, FMD antibody, MNS antibody, OPD antibody, ABPX antibody, CSBS antibody, CVD1 antibody, FLN1 antibody, NHBP antibody, OPD1 antibody, OPD2 antibody, XLVD antibody, XMVD antibody, FLN-A antibody, ABP-280 antibody
ABP 280 antibody|ABP-280 antibody|Actin-binding protein 280 antibody|Alpha filamin antibody|Alpha-filamin antibody|APBX antibody|CSBS antibody|CVD1 antibody|Endothelial actin binding protein antibody|Endothelial actin-binding protein antibody|Filamin 1 antibody|Filamin A alpha antibody|Filamin A antibody|Filamin-1 antibody|Filamin-A antibody|FLN antibody|FLN-A antibody|FLN1 antibody|FLNA antibody|FLNA_HUMAN antibody|FMD antibody|MNS antibody|NHBP antibody|Non muscle filamin antibody|Non-muscle filamin antibody|OPD antibody|OPD1 antibody|OPD2 antibody|XLVD antibody|XMVD antibody|Anti-Filamin A antibody [EP2405Y] (ab76289)
SCBT cat No: sc-58762|sc-71118|sc-135906|sc-17749|sc-58763|sc-271440|sc-28284|sc-7565|sc-58764|
Name | Rabbit polyclonal FLNA antibody |
---|---|
Catalogue No. | A7896 |
Reactivity | Human, Mouse |
Immunogen | A synthetic peptide of human FLNA |
Host | Rabbit |
Applications | WB, IHC |
Recommended dilution | WB 1:500 – 1:2000 |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Isotype | IgG |
Molecular weight | Predicted: 281kDa/Observed: Refer to figures |
Formulation | FLNA antibody was tube-contained. |
Purification | FLNA antibody was purified using affinity purification. |
Storage | Store at -20 Celsius degree. Avoid freeze / thaw cycles. |
Alternative antibody names | FLN antibody, FMD antibody, MNS antibody, OPD antibody, ABPX antibody, CSBS antibody, CVD1 antibody, FLN1 antibody, NHBP antibody, OPD1 antibody, OPD2 antibody, XLVD antibody, XMVD antibody, FLN-A antibody, ABP-280 antibody |
Database links | Human UniProt/Swiss-Prot:P21333 |
Protein names | FLN, FMD, MNS, OPD, ABPX, CSBS, CVD1, FLN1, NHBP, OPD1, OPD2, XLVD, XMVD, FLN-A, ABP-280 |
Protein function | Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintainance (By similarity). |
Protein tissue specificity | Ubiquitous. |
Protein sequence and domain | Comprised of a NH2-terminal actin-binding domain, 24 immunoglobulin-like internally homologous repeats and two hinge regions. Repeat 24 and the second hinge domain are important for dimer formation. Filamin repeat 20 interacts with filamin repeat 21 masking the ligand binding site on filamin repeat 21, resulting in an autoinhibited conformation . The autoinhibition can be relieved by ligands like ITGB7 or FBLIM1 . Filamin repeats 19 and 21 can simultaneously engage ligands .Belongs to the filamin family.; Contains 1 actin-binding domain.; Contains 2 CH (calponin-homology) domains.; Contains 24 filamin repeats. |
Protein post-translational modifications | Phosphorylation at Ser-2152 is negatively regulated by the autoinhibited conformation of filamin repeats 19-21. Ligand binding induces a conformational switch triggering phosphorylation at Ser-2152 by PKA.; Phosphorylation extent changes in response to cell activation.; Polyubiquitination in the CH1 domain by a SCF-like complex containing ASB2 leads to proteasomal degradation. Prior dissociation from actin may be required to expose the target lysines . Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome . |
Protein cellular localization | Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. |
Background | The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene. |
Research area | All research areas>Structural Proteins>Filamin |
Note | AntibodyPlus can customize FLNA antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation. |
Product type | Primary antibody |
Supplier | ABclonal Inc. |
Antibody customer reviews & validation data - 111
Figure description:
Detection of FLNA by immunoblotting in total lysates of hTERT-RPE1 cells
Product name
Rabbit polyclonal FLNA antibody
Product catalog No.
A7896
Product type
Primary antibody
Supplier
ABclonal
Application type
Western Blot
Antibody specificity rating
Good
Antibody overall rating
Good
Your comments
On Western blots with human retinal epithelial cells, the FLNA antibody detected a strong band at >250kDa, in addition to many fainter bands of smaller molecular weight. Since the expected size is 281 kDa, the antibody is apparently specific.
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