Validated Anti-FLNA antibody | Antibodyplus: Antibody Trial and Validation

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Rabbit polyclonal FLNA antibody (A7896)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

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Alternative names:

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SCBT cat No: sc-58762|sc-71118|sc-135906|sc-17749|sc-58763|sc-271440|sc-28284|sc-7565|sc-58764|

Name	Rabbit polyclonal FLNA antibody
Catalogue No.	A7896
Reactivity	Human, Mouse
Immunogen	A synthetic peptide of human FLNA
Host	Rabbit
Applications	WB, IHC
Recommended dilution	WB 1:500 – 1:2000 IHC 1:50 – 1:200
Clonality	Polyclonal
Conjugation	Unconjugated
Isotype	IgG
Molecular weight	Predicted: 281kDa/Observed: Refer to figures
Formulation	FLNA antibody was tube-contained. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification	FLNA antibody was purified using affinity purification.
Storage	Store at -20 Celsius degree. Avoid freeze / thaw cycles.
Alternative antibody names	FLN antibody, FMD antibody, MNS antibody, OPD antibody, ABPX antibody, CSBS antibody, CVD1 antibody, FLN1 antibody, NHBP antibody, OPD1 antibody, OPD2 antibody, XLVD antibody, XMVD antibody, FLN-A antibody, ABP-280 antibody
Database links	Human UniProt/Swiss-Prot:P21333
Protein names	FLN, FMD, MNS, OPD, ABPX, CSBS, CVD1, FLN1, NHBP, OPD1, OPD2, XLVD, XMVD, FLN-A, ABP-280
Protein function	Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintainance (By similarity).
Protein tissue specificity	Ubiquitous.
Protein sequence and domain	Comprised of a NH2-terminal actin-binding domain, 24 immunoglobulin-like internally homologous repeats and two hinge regions. Repeat 24 and the second hinge domain are important for dimer formation. Filamin repeat 20 interacts with filamin repeat 21 masking the ligand binding site on filamin repeat 21, resulting in an autoinhibited conformation . The autoinhibition can be relieved by ligands like ITGB7 or FBLIM1 . Filamin repeats 19 and 21 can simultaneously engage ligands .Belongs to the filamin family.; Contains 1 actin-binding domain.; Contains 2 CH (calponin-homology) domains.; Contains 24 filamin repeats.
Protein post-translational modifications	Phosphorylation at Ser-2152 is negatively regulated by the autoinhibited conformation of filamin repeats 19-21. Ligand binding induces a conformational switch triggering phosphorylation at Ser-2152 by PKA.; Phosphorylation extent changes in response to cell activation.; Polyubiquitination in the CH1 domain by a SCF-like complex containing ASB2 leads to proteasomal degradation. Prior dissociation from actin may be required to expose the target lysines . Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome .
Protein cellular localization	Cytoplasm, cell cortex. Cytoplasm, cytoskeleton.
Background	The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.
Research area	All research areas>Structural Proteins>Filamin (View all antibody categories related to Structural Proteins)
Note	AntibodyPlus can customize FLNA antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.
Product type	Primary antibody
Supplier	ABclonal Inc.

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Antibody customer reviews & validation data - 111

Validation data ID: 111

Submission date: 2017-02-10

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Result figure:

Figure description:

Detection of FLNA by immunoblotting in total lysates of hTERT-RPE1 cells

Product name

Rabbit polyclonal FLNA antibody

Product catalog No.

A7896

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

On Western blots with human retinal epithelial cells, the FLNA antibody detected a strong band at >250kDa, in addition to many fainter bands of smaller molecular weight. Since the expected size is 281 kDa, the antibody is apparently specific.

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Rabbit polyclonal FLNA antibody (A7896)

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