Rabbit polyclonal FLNA antibody (A7896)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal FLNA antibody (A7896)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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FLN antibody, FMD antibody, MNS antibody, OPD antibody, ABPX antibody, CSBS antibody, CVD1 antibody, FLN1 antibody, NHBP antibody, OPD1 antibody, OPD2 antibody, XLVD antibody, XMVD antibody, FLN-A antibody, ABP-280 antibody
ABP 280 antibody|ABP-280 antibody|Actin-binding protein 280 antibody|Alpha filamin antibody|Alpha-filamin antibody|APBX antibody|CSBS antibody|CVD1 antibody|Endothelial actin binding protein antibody|Endothelial actin-binding protein antibody|Filamin 1 antibody|Filamin A alpha antibody|Filamin A antibody|Filamin-1 antibody|Filamin-A antibody|FLN antibody|FLN-A antibody|FLN1 antibody|FLNA antibody|FLNA_HUMAN antibody|FMD antibody|MNS antibody|NHBP antibody|Non muscle filamin antibody|Non-muscle filamin antibody|OPD antibody|OPD1 antibody|OPD2 antibody|XLVD antibody|XMVD antibody|Anti-Filamin A antibody [EP2405Y] (ab76289)

SCBT cat No: sc-58762|sc-71118|sc-135906|sc-17749|sc-58763|sc-271440|sc-28284|sc-7565|sc-58764|

Name

Rabbit polyclonal FLNA antibody

Catalogue No.

A7896

Reactivity

Human, Mouse

Immunogen

A synthetic peptide of human FLNA

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 281kDa/Observed: Refer to figures

Formulation

FLNA antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

FLNA antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

FLN antibody, FMD antibody, MNS antibody, OPD antibody, ABPX antibody, CSBS antibody, CVD1 antibody, FLN1 antibody, NHBP antibody, OPD1 antibody, OPD2 antibody, XLVD antibody, XMVD antibody, FLN-A antibody, ABP-280 antibody

Database links

Human UniProt/Swiss-Prot:P21333

Protein names

FLN, FMD, MNS, OPD, ABPX, CSBS, CVD1, FLN1, NHBP, OPD1, OPD2, XLVD, XMVD, FLN-A, ABP-280

Protein function

Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintainance (By similarity).

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

Comprised of a NH2-terminal actin-binding domain, 24 immunoglobulin-like internally homologous repeats and two hinge regions. Repeat 24 and the second hinge domain are important for dimer formation. Filamin repeat 20 interacts with filamin repeat 21 masking the ligand binding site on filamin repeat 21, resulting in an autoinhibited conformation . The autoinhibition can be relieved by ligands like ITGB7 or FBLIM1 . Filamin repeats 19 and 21 can simultaneously engage ligands .Belongs to the filamin family.; Contains 1 actin-binding domain.; Contains 2 CH (calponin-homology) domains.; Contains 24 filamin repeats.

Protein post-translational modifications

Phosphorylation at Ser-2152 is negatively regulated by the autoinhibited conformation of filamin repeats 19-21. Ligand binding induces a conformational switch triggering phosphorylation at Ser-2152 by PKA.; Phosphorylation extent changes in response to cell activation.; Polyubiquitination in the CH1 domain by a SCF-like complex containing ASB2 leads to proteasomal degradation. Prior dissociation from actin may be required to expose the target lysines . Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome .

Protein cellular localization

Cytoplasm, cell cortex. Cytoplasm, cytoskeleton.

Background

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.

Research area

All research areas>Structural Proteins>Filamin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize FLNA antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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Antibody customer reviews & validation data - 111

Validation data ID: 111

Submission date: 2017-02-10

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Result figure:
Result figure

Figure description:

Detection of FLNA by immunoblotting in total lysates of hTERT-RPE1 cells

Product name

Rabbit polyclonal FLNA antibody

Product catalog No.

A7896

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

On Western blots with human retinal epithelial cells, the FLNA antibody detected a strong band at >250kDa, in addition to many fainter bands of smaller molecular weight. Since the expected size is 281 kDa, the antibody is apparently specific.

Detailed protocol

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