Rabbit polyclonal FOXO1 antibody (A2934)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal FOXO1 antibody (A2934)

Supplier: ABclonal Inc.

Recommended applications: WB,IF

Recommended dilution:

WB 1:500 – 1:2000 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal FOXO1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

FKH1 antibody, FKHR antibody, FOXO1A antibody
FKH 1 antibody|FKH1 antibody|FKHR antibody|Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody|Forkhead box O1 antibody|Forkhead box protein O1 antibody|Forkhead box protein O1A antibody|Forkhead in rhabdomyosarcoma antibody|Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody|FoxO transcription factor antibody|foxo1 antibody|FOXO1_HUMAN antibody|FOXO1A antibody|OTTHUMP00000018301 antibody|Anti-FOXO1A antibody – ChIP Grade (ab39670)

SCBT cat No: sc-49437|sc-373901|sc-6430|sc-6432|sc-11409|sc-6431|sc-32253|sc-32252|sc-1442|sc-292790|sc-58603|sc-58604|sc-368613|sc-18277|sc-5589|sc-365251|

Name

Rabbit polyclonal FOXO1 antibody

Catalogue No.

A2934

Reactivity

Human, Mouse

Immunogen

A synthetic peptide of human FOXO1

Host

Rabbit

Applications

WB, IF

Recommended dilution

WB 1:500 – 1:2000
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 70kDa/Observed: Refer to Figures

Formulation

FOXO1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

FOXO1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

FKH1 antibody, FKHR antibody, FOXO1A antibody

Database links

Human UniProt/Swiss-Prot:Q12778

Protein names

FKH1, FKHR, FOXO1A

Protein function

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5′-TT[G/A]TTTTG-3′ and the related Daf-16 family binding element (DBE) with consensus sequence 5′-TT[G/A]TTTAC-3′. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner.

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

Contains 1 fork-head DNA-binding domain.

Protein post-translational modifications

Phosphorylation by NLK promotes nuclear export and inhibits the transcriptional activity. In response to growth factors, phosphorylation on Thr-24, Ser-256 and Ser-322 by PKB/AKT1 promotes nuclear export and inactivation of transactivational activity. Phosphorylation on Thr-24 is required for binding 14-3-3 proteins. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24 and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CDK1, leading to nuclear exclusion and loss of function. Stress signals, such as response to oxygen or nitric oxide, attenuate the PKB/AKT1-mediated phosphorylation leading to nuclear retention. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Dephosphorylated on Thr-24 and Ser-256 by PP2A in beta-cells under oxidative stress leading to nuclear retention (By similarity). Phosphorylation of Ser-249 by CDK1 disrupts binding of 14-3-3 proteins leading to nuclear accumulation and has no effect on DNA-binding nor transcriptional activity. Phosphorylation by STK4/MST1 on Ser-212, upon oxidative stress, inhibits binding to 14-3-3 proteins and nuclear export.; Acetylated. Acetylation at Lys-262, Lys-265 and Lys-274 are necessary for autophagic cell death induction. Deacetylated by SIRT2 in response to oxidative stress or serum deprivation, thereby negatively regulating FOXO1-mediated autophagic cell death.; Ubiquitinated by SRT2. Ubiquitination leads to proteasomal degradation.; Methylation inhibits AKT1-mediated phosphorylation at Ser-256 and is increased by oxidative stress.; Once in the nucleus, acetylated by CREBBP/EP300. Acetylation diminishes the interaction with target DNA and attenuates the transcriptional activity. It increases the phosphorylation at Ser-256. Deacetylation by SIRT1 results in reactivation of the transcriptional activity. Oxidative stress by hydrogen peroxide treatment appears to promote deacetylation and uncoupling of insulin-induced phosphorylation. By contrast, resveratrol acts independently of acetylation.

Protein cellular localization

Cytoplasm . Nucleus . Note: Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway. Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1. SGK1-mediated phosphorylation also results in nuclear translocation. Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide. Retained in the nucleus on methylation.

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Interacts with LRPPRC. Interacts with RUNX2; the interaction inhibits RUNX2 transcriptional activity and mediates the IGF1/insulin-dependent BGLAP expression in osteoblasts Interacts with PPP2R1A; the interaction regulates the dephosphorylation of FOXO1 at Thr-24 and Ser-256 leading to its nuclear import.

Research area

All research areas>Transcription Regulators>FKHR
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize FOXO1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!