Rabbit polyclonal H2AFY antibody (A7045)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal H2AFY antibody (A7045)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal H2AFY antibody.

Alternative names:

Check alternative names for the antibody

Expand

H2A.y antibody, H2A/y antibody, H2AFJ antibody, mH2A1 antibody, H2AF12M antibody, MACROH2A1.1 antibody, macroH2A1.2 antibody
Core histone macro h2a.1 antibody|Core histone macro-H2A.1 antibody|H2A histone family member Y antibody|H2A.y antibody|H2A/y antibody|H2AF12M antibody|H2AFJ antibody|H2afy antibody|H2AY_HUMAN antibody|Histone H2A.Y antibody|Histone macroH2A1 antibody|Histone macroH2A1.1 antibody|Histone macroH2A1.2 antibody|Macroh2a1 antibody|MACROH2A1.1 antibody|MacroH2A1.2 antibody|Medulloblastoma antigen MU MB 50.205 antibody|Medulloblastoma antigen MU-MB-50.205 antibody|mH2a antibody|mH2A1 antibody|Anti-macroH2A.1 antibody – ChIP Grade (ab37264)

SCBT cat No:

Name

Rabbit polyclonal H2AFY antibody

Catalogue No.

A7045

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human H2AFY

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 40kDa/Observed: Refer to figures

Formulation

H2AFY antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

H2AFY antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

H2A.y antibody, H2A/y antibody, H2AFJ antibody, mH2A1 antibody, H2AF12M antibody, MACROH2A1.1 antibody, macroH2A1.2 antibody

Database links

Human UniProt/Swiss-Prot:O75367

Protein names

H2A.y, H2A/y, H2AFJ, mH2A1, H2AF12M, MACROH2A1.1, macroH2A1.2

Protein function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

Contains 1 histone H2A domain.; Contains 1 Macro domain.

Protein post-translational modifications

Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.

Protein cellular localization

Nucleus . Chromosome . Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Note

AntibodyPlus can customize H2AFY antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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Antibody customer reviews & validation data - 148

Validation data ID: 148

Submission date: 2017-03-14

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Result figure:
Result figure

Figure description:

Nuclear distribution of H2AFY (red) at interphase in primary female fibroblast cell.

Product name

Rabbit polyclonal H2AFY antibody

Product catalog No.

A7045

Product type

Primary antibody

Supplier

ABclonal

Application type

Immunocytochemistry (ICC)

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

The antibody worked fine and X chromosome is clearly visible.

Detailed protocol

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