Rabbit polyclonal HCFC1 antibody (A1841)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal HCFC1 antibody (A1841)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal HCFC1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

CFF antibody, HCF1 antibody, HFC1 antibody, MRX3 antibody, VCAF antibody, HCF-1 antibody
C1 factor antibody|CFF antibody|HCF 1 antibody|HCF antibody|HCF C-terminal chain 6 antibody|HCF-1 antibody|HCF1 antibody|Hcfc1 antibody|HCFC1_HUMAN antibody|HFC1 antibody|Host cell factor 1 antibody|Host cell factor antibody|Host cell factor C1 (VP16 accessory protein) antibody|Host cell factor C1 antibody|MGC70925 antibody|MRX3 antibody|VCAF antibody|VP16 accessory protein antibody|Anti-HCF-1 / Host Cell Factor C1 antibody – N-terminal (ab137618)

SCBT cat No: sc-13429|sc-390950|sc-13427|

Name

Rabbit polyclonal HCFC1 antibody

Catalogue No.

A1841

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human HCFC1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 209kDa/Observed: Refer to Figures

Formulation

HCFC1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

HCFC1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CFF antibody, HCF1 antibody, HFC1 antibody, MRX3 antibody, VCAF antibody, HCF-1 antibody

Database links

Human UniProt/Swiss-Prot:P51610

Protein names

CFF, HCF1, HFC1, MRX3, VCAF, HCF-1

Protein function

Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 ‘Lys-4’ methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.

Protein tissue specificity

Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested.

Protein sequence and domain

The HCF repeat is a highly specific proteolytic cleavage signal.; The kelch repeats fold into a 6-bladed kelch beta-propeller called the beta-propeller domain which mediates interaction with HCFC1R1.Contains 3 fibronectin type-III domains.; Contains 5 Kelch repeats.

Protein post-translational modifications

Proteolytically cleaved at one or several PPCE–THET sites within the HCF repeats. Further cleavage of the primary N- and C-terminal chains results in a ‘trimming’ and accumulation of the smaller chains. Cleavage is promoted by O-glycosylation.; O-glycosylated. GlcNAcylation by OGT promotes proteolytic processing.; Ubiquitinated. Lys-1807 and Lys-1808 are ubiquitinated both via ‘Lys-48’- and ‘Lys-63’-linked polyubiquitin chains. BAP1 mediated deubiquitination of ‘Lys-48’-linked polyubiquitin chains; deubiquitination by BAP1 does not seem to stabilize the protein.

Protein cellular localization

Cytoplasm. Nucleus. Note: HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. Nuclear in general, but uniquely cytoplasmic in trigeminal ganglia, becoming nuclear upon HSV reactivation from the latent state. Non-processed HCFC1 associates with chromatin. Colocalizes with CREB3 and CANX in the ER.

Background

This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Research area

All research areas>Transcription Regulators>HCF
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize HCFC1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!