Rabbit polyclonal HDAC4 antibody (A0239)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF,IP,CHIP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal HDAC4 antibody (A0239)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF,IP,CHIP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:50 – 1:200 ChIP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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HDAC4 antibody,HA6116 antibody,HD4 antibody,HDAC-A antibody,HDACA antibody,KIAA0288 antibody
AHO3 antibody|MEF2-interacting transcription repressor MITR antibody|MITR antibody|NY-CO-9 antibody|Anti-HDAC4 antibody (ab12172)

SCBT cat No: sc-46672|sc-365093|sc-48390|sc-365367|sc-11418|sc-56686|sc-5246|sc-5245|sc-11421|

Name

Rabbit polyclonal HDAC4 antibody

Catalogue No.

A0239

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human HDAC4

Host

Rabbit

Applications

WB, IHC, IF, IP, CHIP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:50 – 1:200
ChIP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 119kDa/Observed: Refer to Figures

Formulation

HDAC4 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

HDAC4 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HDAC4 antibody,HA6116 antibody,HD4 antibody,HDAC-A antibody,HDACA antibody,KIAA0288 antibody

Database links

Human UniProt/Swiss-Prot:P56524

Protein names

HDAC4,HA6116,HD4,HDAC-A,HDACA,KIAA0288

Protein function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.Belongs to the histone deacetylase family. HD type 2 subfamily.

Protein post-translational modifications

Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350 impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3.; Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.

Protein cellular localization

Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation.

Background

Acetylation of the histone tail causes chromatin to adopt an “open” conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a “closed” chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

Research area

All research areas>Transcription Regulators>HDAC
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize HDAC4 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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