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Rabbit polyclonal HIST1H2AG antibody (A9895)
Supplier: ABclonal Inc.
Recommended applications: WB
WB 1:500 – 1:2000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
H2a 615 antibody|H2A antibody|H2A GL101 antibody|H2A histone family member A antibody|H2A.1 antibody|H2A.2 antibody|H2A/a antibody|H2A/m antibody|H2A/O antibody|H2A/q antibody|H2A1B_HUMAN antibody|H2AFA antibody|H2AFE antibody|H2AFL antibody|H2AFM antibody|H2AFO antibody|H2AFQ antibody|HIST1H2AE antibody|HIST1H2AJ antibody|HIST2H2AA antibody|HIST2H2AA3 antibody|HIST2H2AB antibody|HIST2H2AC antibody|Histone 1 H2ae antibody|Histone 2 H2aa3 antibody|Histone 2 H2ab antibody|Histone 2 H2ac antibody|Histone H2A type 1 B antibody|Histone H2A type 1 C antibody|Histone H2A type 1 E antibody|Histone H2A type 1 J antibody|Histone H2A type 1-B/E antibody|Histone H2A.2 antibody|Histone H2A/a antibody|Histone H2A/m antibody|MGC74460 antibody|Anti-Histone H2A antibody (ab18255) ChIP Grade
SCBT cat No: sc-133655|
Rabbit polyclonal HIST1H2AG antibody
|Catalogue No.|| |
Recombinant protein of human HIST1H2AG
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 14kDa/Observed: Refer to figures
HIST1H2AG antibody was tube-contained.
HIST1H2AG antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Database links|| |
|Protein function|| |
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
|Protein sequence and domain|| |
Belongs to the histone H2A family.
|Protein post-translational modifications|| |
Deiminated on Arg-4 in granulocytes upon calcium entry.; Monoubiquitination of Lys-120 (H2AK119Ub) by RING1, TRIM27 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of ‘Lys-27’ of histone H3 (H3K27me). H2AK119Ub by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Monoubiquitination of Lys-120 (H2AK119Ub) by TRIM27 may promote transformation of cells in a number of breast cancers . Following DNA double-strand breaks (DSBs), it is ubiquitinated through ‘Lys-63’ linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Ubiquitination at Lys-14 and Lys-16 (H2AK13Ub and H2AK15Ub, respectively) in response to DNA damage is initiated by RNF168 that mediates monoubiquitination at these 2 sites, and ‘Lys-63’-linked ubiquitin are then conjugated to monoubiquitin; RNF8 is able to extend ‘Lys-63’-linked ubiquitin chains in vitro. H2AK119Ub and ionizing radiation-induced ‘Lys-63’-linked ubiquitination (H2AK13Ub and H2AK15Ub) are distinct events.; Phosphorylation on Ser-2 (H2AS1ph) is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1. Phosphorylation at Thr-121 (H2AT120ph) by VPRBP is present in the regulatory region of many tumor suppresor genes and down-regulates their transcription.; Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.; Glutamine methylation at Gln-105 (H2AQ104me) by FBL is specifically dedicated to polymerase I. It is present at 35S ribosomal DNA locus and impairs binding of the FACT complex .; Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
|Protein cellular localization|| |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]
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|Product type|| |
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