Rabbit Polyclonal Histone H2A antibody (STJ96865)

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Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Histone H2A antibody (STJ96865)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

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Alternative names:

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HIST1H2AG antibody, H2AFP; antibody, HIST1H2AI antibody, H2AFC; antibody, HIST1H2AK antibody, H2AFD; antibody, HIST1H2AL antibody, H2AFI; antibody, HIST1H2AM antibody, H2AFN antibody,|H2a 615 antibody|H2A antibody|H2A GL101 antibody|H2A histone family member A antibody|H2A.1 antibody|H2A.2 antibody|H2A/a antibody|H2A/m antibody|H2A/O antibody|H2A/q antibody|H2A1B_HUMAN antibody|H2AFA antibody|H2AFE antibody|H2AFL antibody|H2AFM antibody|H2AFO antibody|H2AFQ antibody|HIST1H2AE antibody|HIST1H2AJ antibody|HIST2H2AA antibody|HIST2H2AA3 antibody|HIST2H2AB antibody|HIST2H2AC antibody|Histone 1 H2ae antibody|Histone 2 H2aa3 antibody|Histone 2 H2ab antibody|Histone 2 H2ac antibody|Histone H2A type 1 B antibody|Histone H2A type 1 C antibody|Histone H2A type 1 E antibody|Histone H2A type 1 J antibody|Histone H2A type 1-B/E antibody|Histone H2A.2 antibody|Histone H2A/a antibody|Histone H2A/m antibody|MGC74460 antibody|Anti-Histone H2A antibody (ab18255) ChIP Grade
SCBT cat No: sc-133655|

Name

Histone H2A Polyclonal Antibody

Catalogue No.

STJ96865

Reactivity

Human, Mouse, Rat

Specificity

Histone H2A Polyclonal Antibody detects endogenous levels of Histone H2A protein.

Immunogen

Synthesized peptide derived from the Internal region of human Histone H2A

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Formulation

Histone H2A Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Histone H2A Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Histone H2A type 1 antibody, H2A.1 antibody, Histone H2A/p antibody

Database links

Human UniProt/Swiss-Prot:P0C0S8;Mouse UniPort/Swiss-Prot: P22752;Rat UniProt/Swiss-Port: P0C170;Human Entrez Gene: 8329;Mouse Entrez Gene: 319170;

Protein names

Histone H2A type 1 , H2A.1 , Histone H2A/p

Protein function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Protein sequence and domain

Belongs to the histone H2A family.

Protein post-translational modifications

Deiminated on Arg-4 in granulocytes upon calcium entry. / Monoubiquitination of Lys-120 (H2AK119Ub) by RING1, TRIM27 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of ‘Lys-27’ of histone H3 (H3K27me). H2AK119Ub by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Monoubiquitination of Lys-120 (H2AK119Ub) by TRIM27 may promote transformation of cells in a number of breast cancers . Following DNA double-strand breaks (DSBs), it is ubiquitinated through ‘Lys-63’ linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Ubiquitination at Lys-14 and Lys-16 (H2AK13Ub and H2AK15Ub, respectively) in response to DNA damage is initiated by RNF168 that mediates monoubiquitination at these 2 sites, and ‘Lys-63’-linked ubiquitin are then conjugated to monoubiquitin; RNF8 is able to extend ‘Lys-63’-linked ubiquitin chains in vitro. H2AK119Ub and ionizing radiation-induced ‘Lys-63’-linked ubiquitination (H2AK13Ub and H2AK15Ub) are distinct events. / Phosphorylation on Ser-2 (H2AS1ph) is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1. Phosphorylation at Thr-121 (H2AT120ph) by VPRBP is present in the regulatory region of many tumor suppresor genes and down-regulates their transcription. / Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. / Glutamine methylation at Gln-105 (H2AQ104me) by FBL is specifically dedicated to polymerase I. It is present at 35S ribosomal DNA locus and impairs binding of the FACT complex . / Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Protein cellular localization

Nucleus / Chromosome

Research area

All research areas>Transcription Regulators>Histone
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Histone H2A Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: Histone H2A Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: Histone H2A Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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