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Rabbit Polyclonal Histone H3 (Acetyl Lys14) antibody (STJ97144)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB
Recommended dilution: WB 1:500-1000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
HIST1H3A antibody, H3FA; antibody, HIST1H3B antibody, H3FL; antibody, HIST1H3C antibody, H3FC; antibody, HIST1H3D antibody, H3FB; antibody, HIST1H3E antibody, H3FD; antibody, HIST1H3F antibody, H3FI; antibody, HIST1H3G antibody, H3FH; antibody, HIST1H3H antibody, H3FK; antibody, HIST1H3I antibody, H3FF; antibody, HIST1H3J antibody, H3FJ antibody,|H3 histone family, member A antibody|H3/A antibody|H31_HUMAN antibody|H3FA antibody|Hist1h3a antibody|HIST1H3B antibody|HIST1H3C antibody|HIST1H3D antibody|HIST1H3E antibody|HIST1H3F antibody|HIST1H3G antibody|HIST1H3H antibody|HIST1H3I antibody|HIST1H3J antibody|histone 1, H3a antibody|Histone cluster 1, H3a antibody|Histone H3.1 antibody|Histone H3/a antibody|Histone H3/b antibody|Histone H3/c antibody|Histone H3/d antibody|Histone H3/f antibody|Histone H3/h antibody|Histone H3/i antibody|Histone H3/j antibody|Histone H3/k antibody|Histone H3/l antibody|Anti-Histone H3 antibody (ab1791) ChIP Grade
SCBT cat No: sc-52942|sc-56745|
Histone H3 (Acetyl Lys14) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
The antibody detects endogenous Histone H3 (Acetyl Lys14) protein.
|Recommended dilution|| |
Histone H3 (Acetyl Lys14) Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Histone H3 (Acetyl Lys14) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody
|Database links|| |
Human UniProt/Swiss-Prot:<a href="http://www.uniprot.org/uniprot/P68431">P68431 </a>;Human Entrez Gene: <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8350">8350</a>;
|Protein names|| |
Histone H3.1 , Histone H3/a , Histone H3/b , Histone H3/c , Histone H3/d , Histone H3/f , Histone H3/h , Histone H3/i , Histone H3/j , Histone H3/k , Histone H3/l
|Protein function|| |
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
|Protein tissue specificity|| |
Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
|Protein sequence and domain|| |
Belongs to the histone H3 family.
|Protein post-translational modifications|| |
Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. / Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription. / Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters. / Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication. / Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Thr-12 (H3T11ph) by chromatin-associated CHEK1 regulates the transcription of cell cycle regulatory genes by modulating acetylation of Lys-10 (H3K9ac). Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin. / Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. / Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression . / Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
|Protein cellular localization|| |
Nucleus / Chromosome
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/Histone/">Histone</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"> (View all antibody categories related to Transcription Regulators)</a>
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St John’s Laboratory Ltd.
|Product type|| |
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Antibody customer reviews & validation data - 25
Validation data ID: 25
Submission date: 2016-11-25
Rabbit polyclonal Histone H3 (Acetyle Lys14) antibody
Product catalog No.
St John's Laboratory Ltd
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