Rabbit Polyclonal Histone H4 (Acetyl Lys5) antibody (STJ97179)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Histone H4 (Acetyl Lys5) antibody (STJ97179)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:2000-5000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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HIST1H4A antibody, H4/A antibody, H4FA; antibody, HIST1H4B antibody, H4/I antibody, H4FI; antibody, HIST1H4C antibody, H4/G antibody, H4FG; antibody, HIST1H4D antibody, H4/B antibody, H4FB; antibody, HIST1H4E antibody, H4/J antibody, H4FJ; antibody, HIST1H4F antibody, H4/C antibody, H4FC; antibody, HIST1H4H antibody, H4/H antibody, H4FH; antibody, HIST1H4I antibody, H4/M antibody, H4FM; antibody, HIST1H4J antibody, H4/E antibody, H4FE; antibody, HIST1H4K antibody, H4/D antibody, H4FD; antibody, HIST1H4L antibody, H4/K antibody, H4FK; antibody, HIST2H4A antibody, H4/N antibody, H4F2 antibody, H4FN antibody, HIST2H4; antibody, HIST2H4B antibody, H4/O antibody, H4FO; antibody, HIST4H4 antibody,|H4_HUMAN antibody|H4F2 antibody|H4FN antibody|HIST1H4 antibody|HIST2H4 antibody|Hist4h4 antibody|HISTH4H4 antibody|Histone H4 antibody|methyl histone H4 antibody|methylated histone H4 antibody|Anti-Histone H4K20me1 Antibody – ChIP Grade (ab9051)
SCBT cat No: To be updated

 

Name

Histone H4 (Acetyl Lys5) Polyclonal Antibody

Catalogue No.

STJ97179

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous Histone H3 (Acetyl Lys5) protein.

Immunogen

Synthetic Peptide

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:2000-5000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

Histone H4 (Acetyl Lys5) Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

Histone H4 (Acetyl Lys5) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Histone H4 antibody

Database links

Human UniProt/Swiss-Prot:P62805;Mouse UniPort/Swiss-Prot: P62806;Human Entrez Gene: 121504;Mouse Entrez Gene: 319156;

Protein names

Histone H4

Protein function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Involvement in disease

Note: Chromosomal aberrations involving HISTONE H4 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;6)(q27;p21), with BCL6.

Protein sequence and domain

Belongs to the histone H4 family.

Protein post-translational modifications

Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. / Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. / Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. / Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by KMT5B and KMT5C and induces gene silencing. / Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. / Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). / Sumoylated, which is associated with transcriptional repression. / Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Protein cellular localization

Nucleus / Chromosome

Research area

All research areas>Transcription Regulators>Histone
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Histone H4 (Acetyl Lys5) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 23

Validation data ID: 23

Submission date: 2016-11-25

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Result figure:
Result figure

Figure description:

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Product name

Rabbit polyclonal Histone H4 (Acetyle Lys5) antibody

Product catalog No.

STJ97179

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

-

Detailed protocol

View Details

Antibody customer reviews & validation data - 12

Validation data ID: 12

Submission date: 2016-11-18

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Result figure:
Result figure

Figure description:

When tested with MCF7 cell lysate, STJ97179 showed a single specific band with strong signal.

Product name

Rabbit polyclonal Histone H4 (Acetyle Lys5) antibody

Product catalog No.

STJ97179

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

Excellent performance!

Detailed protocol

View Details

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