Rabbit polyclonal HLA-DQA1 antibody (A2168)

$69.00$259.00

Reactivity: Human
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal HLA-DQA1 antibody (A2168)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

HLA-DQA1 antibody, CD antibody, CELIAC1 antibody, DQ-A1 antibody, GSE antibody, HLA-DQA antibody, DQ alpha 1 chain antibody, DC-1 alpha chain antibody, DC-alpha antibody, HLA-DCA antibody, MHC class II DQA1 antibody
CD antibody|CELIAC 1 antibody|CELIAC1 antibody|DP beta 1 chain antibody|DP(W4) beta chain antibody|DPB1 antibody|DPB1_HUMAN antibody|DQ A1 antibody|DRB1 antibody|DRB4 antibody|FLJ27088 antibody|FLJ27328 antibody|GSE antibody|HLA class II histocompatibility antigen antibody|HLA class II histocompatibility antigen DR 1 beta antibody|HLA class II histocompatibility antigen DR alpha antibody|HLA class II histocompatibility antigen DR alpha chain antibody|HLA DP histocompatibility type beta 1 subunit antibody|HLA DP1B antibody|HLA DPB1 antibody|HLA DQA antibody|HLA DQA1 antibody|HLA DQB antibody|HLA DQB1 antibody|HLA DR1B antibody|HLA DR3B antibody|HLA DR4B antibody|HLA DRA antibody|HLA DRA1 antibody|HLA DRB1 antibody|HLA DRB3 antibody|HLA DRB4 antibody|HLA DRB5 antibody|HLA-DPB1 antibody|IDDM 1 antibody|IDDM1 antibody|Major histocompatibility complex class II DP beta 1 antibody|Major histocompatibility complex class II DQ alpha 1 antibody|Major histocompatibility complex class II DQ beta 1 antibody|Major histocompatibility complex class II DR alpha antibody|Major histocompatibility complex class II DR beta 1 antibody|Major histocompatibility complex class II DR beta 3 antibody|Major histocompatibility complex class II DR beta 4 antibody|Major histocompatibility complex class II DR beta 5 antibody|MGC117330 antibody|MHC class II antigen DPB1 antibody|MHC class II antigen DRA antibody|MHC class II HLA DQ alpha 1 antibody|MHC class II HLA DR beta 1 antibody|MHC class II HLA DR beta 3 antibody|MHC DPB1 antibody|MHC DQ beta antibody|MHC HLA DPB1 antibody|MHC HLA DQ alpha antibody|Anti-HLA-DQA1 antibody [EPR7300] (ab128959)

SCBT cat No: sc-59239|sc-65320|

Name

Rabbit polyclonal HLA-DQA1 antibody

Catalogue No.

A2168

Reactivity

Human

Immunogen

Recombinant protein of human HLA-DQA1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 28kDa/Observed: Refer to Figures

Formulation

HLA-DQA1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

HLA-DQA1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HLA-DQA1 antibody, CD antibody, CELIAC1 antibody, DQ-A1 antibody, GSE antibody, HLA-DQA antibody, DQ alpha 1 chain antibody, DC-1 alpha chain antibody, DC-alpha antibody, HLA-DCA antibody, MHC class II DQA1 antibody

Database links

Human UniProt/Swiss-Prot:P01909

Protein names

HLA-DQA1, CD, CELIAC1, DQ-A1, GSE, HLA-DQA, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1

Protein function

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Protein sequence and domain

Belongs to the MHC class II family.; Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Protein cellular localization

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

Background

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules (1).1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.

Research area

All research areas>Lymphocyte Signaling>HLA
(View all antibody categories related to Lymphocyte Signaling)

Note

AntibodyPlus can customize HLA-DQA1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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