Rabbit polyclonal HLA-DRA antibody (A1579)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal HLA-DRA antibody (A1579)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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MLRW antibody, HLA-DRA1 antibody
DR alpha chain antibody|DR alpha chain precursor antibody|DRA_HUMAN antibody|DRB1 antibody|DRB4 antibody|Histocompatibility antigen HLA DR alpha antibody|HLA class II histocompatibility antigen antibody|HLA class II histocompatibility antigen DR alpha chain antibody|HLA DR1B antibody|HLA DR3B antibody|HLA DRA antibody|HLA DRA1 antibody|HLA DRB1 antibody|HLA DRB3 antibody|HLA DRB4 antibody|HLA DRB5 antibody|HLA-DRA antibody|HLADR4B antibody|HLADRA1 antibody|HLADRB antibody|Major histocompatibility complex class II DR alpha antibody|Major histocompatibility complex class II DR beta 1 antibody|Major histocompatibility complex class II DR beta 3 antibody|Major histocompatibility complex class II DR beta 4 antibody|Major histocompatibility complex class II DR beta 5 antibody|MGC117330 antibody|MHC cell surface glycoprotein antibody|MHC class II antigen DRA antibody|MHC II antibody|MLRW antibody|Anti-HLA DR antibody [EPR3692] (ab92511)

SCBT cat No: sc-53305|sc-32248|sc-14532|sc-292171|sc-14536|sc-14537|sc-134356|sc-69738|sc-393548|sc-14539|sc-515446|sc-103546|sc-69739|sc-14546|

Name

Rabbit polyclonal HLA-DRA antibody

Catalogue No.

A1579

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human HLA-DRA

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 29kDa/Observed: Refer to Figures

Formulation

HLA-DRA antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

HLA-DRA antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MLRW antibody, HLA-DRA1 antibody

Database links

Human UniProt/Swiss-Prot:P01903

Protein names

MLRW, HLA-DRA1

Protein function

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Protein sequence and domain

Belongs to the MHC class II family.; Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Protein post-translational modifications

Ubiquitinated by MARCH1 or MARCH8 at Lys-244 leading to down-regulation of MHC class II. When associated with ubiquitination of the beta subunit of HLA-DR: HLA-DRB4 ‘Lys-254’, the down-regulation of MHC class II may be highly effective.

Protein cellular localization

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Note: The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

Background

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq, Jul 2008]

Research area

All research areas>Lymphocyte Signaling>HLA
(View all antibody categories related to Lymphocyte Signaling)

Note

AntibodyPlus can customize HLA-DRA antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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