Rabbit polyclonal HLA-DRB1 antibody (A7685)

$69.00$259.00

Reactivity: Human
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal HLA-DRB1 antibody (A7685)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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SS1 antibody, DRB1 antibody, DRw10 antibody, HLA-DRB antibody, HLA-DR1B antibody
2B1F_HUMAN antibody|DR1 antibody|DR16 antibody|DR5 antibody|DR8 antibody|DRB1 antibody|DRw10 antibody|DW2.2/DR2.2 antibody|FLJ75017 antibody|FLJ76359 antibody|HLA class II antigen beta chain antibody|HLA class II histocompatibility antigen, DR-1 beta chain antibody|HLA class II histocompatibility antigen, DRB1-15 beta chain antibody|HLA-DR-beta 1 antibody|HLA-DR1B antibody|HLA-DRB antibody|HLA-DRB1 antibody|human leucocyte antigen DRB1 antibody|Leucocyte antigen DR beta 1 chain antibody|leucocyte antigen DRB1 antibody|lymphocyte antigen DRB1 antibody|major histocompatibility complex, class II, DR beta 1 antibody|MHC class II antigen antibody|MHC class II antigen DRB1*15 antibody|MHC class II antigen HLA-DR13 antibody|MHC class II HLA-DR beta 1 chain antibody|MHC class II HLA-DR-beta cell surface glycoprotein antibody|MHC class II HLA-DRw10-beta antibody|SS1 antibody|Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

SCBT cat No:

Name

Rabbit polyclonal HLA-DRB1 antibody

Catalogue No.

A7685

Reactivity

Human

Immunogen

Recombinant protein of human HLA-DRB1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 30kDa/Observed: Refer to figures

Formulation

HLA-DRB1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

HLA-DRB1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

SS1 antibody, DRB1 antibody, DRw10 antibody, HLA-DRB antibody, HLA-DR1B antibody

Database links

Human UniProt/Swiss-Prot:P04229

Protein names

SS1, DRB1, DRw10, HLA-DRB, HLA-DR1B

Protein function

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading.; (Microbial infection) Acts as a receptor for Epstein-Barr virus on lymphocytes.

Protein sequence and domain

Belongs to the MHC class II family.; Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Protein post-translational modifications

Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to sorting into the endosome system and down-regulation of MHC class II.

Protein cellular localization

Cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Golgi apparatus, trans-Golgi network membrane ; Single-pass type I membrane protein . Endosome membrane ; Single-pass type I membrane protein . Lysosome membrane ; Single-pass type I membrane protein . Late endosome membrane ; Single-pass type I membrane protein . Note: The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

Background

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

Note

AntibodyPlus can customize HLA-DRB1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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