Rabbit polyclonal HUS1 antibody (A5407)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal HUS1 antibody (A5407)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal HUS1 antibody.

Alternative names:

Check alternative names for the antibody

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hHUS1 antibody
Checkpoint protein HUS1 antibody|hHUS1 antibody|HUS1 (S. pombe) checkpoint homolog antibody|Hus1 antibody|HUS1 checkpoint homolog (S. pombe) antibody|HUS1 Checkpoint Protein antibody|HUS1 protein antibody|HUS1+ – like protein antibody|HUS1_HUMAN antibody|Hydroxyurea-sensitive 1, S. pombe, homolog of antibody|Anti-HUS1 antibody (ab186850)

SCBT cat No: sc-30543|sc-166440|sc-8323|sc-30542|

Name

Rabbit polyclonal HUS1 antibody

Catalogue No.

A5407

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human HUS1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 31kDa/Observed: Refer to Figures

Formulation

HUS1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

HUS1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

hHUS1 antibody

Database links

Human UniProt/Swiss-Prot:O60921

Protein names

hHUS1

Protein function

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3′-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

Belongs to the HUS1 family.

Protein cellular localization

Nucleus . Cytoplasm, cytosol . Note: In discrete nuclear foci upon DNA damage. According to PubMed:11077446, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.

Background

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.

Note

AntibodyPlus can customize HUS1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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