Rabbit polyclonal IFIH1 antibody (A2203)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal IFIH1 antibody (A2203)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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Hlcd antibody, IDDM19 antibody, MDA-5 antibody, MDA5 antibody, MGC133047 antibody
CADM-140 autoantigen antibody|Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody|DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody|DEAD/H box polypeptide antibody|Helicard antibody|Helicase with 2 CARD domains antibody|Hlcd antibody|IDDM 19 antibody|IDDM19 antibody|IFIH 1 antibody|Ifih1 antibody|IFIH1_HUMAN antibody|Interferon induced helicase C domain containing protein 1 antibody|interferon induced with helicase C domain 1 antibody|Interferon induced with helicase C domain protein 1 antibody|Interferon-induced helicase C domain-containing protein 1 antibody|Interferon-induced with helicase C domain protein 1 antibody|MDA 5 antibody|MDA-5 antibody|Melanoma differentiation associated protein 5 antibody|Melanoma differentiation-associated gene 5 antibody|Melanoma differentiation-associated protein 5 antibody|MGC133047 antibody|Murabutide down regulated protein antibody|Murabutide down-regulated protein antibody|RH 116 antibody|RH116 antibody|RIG I like receptor 2 antibody|RLR 2 antibody|RNA helicase DEAD box protein 116 antibody|RNA helicase-DEAD box protein 116 antibody|Anti-MDA5 antibody (ab69983)

SCBT cat No: sc-48031|sc-365630|sc-134513|

Name

Rabbit polyclonal IFIH1 antibody

Catalogue No.

A2203

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human IFIH1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 117kDa/Observed: Refer to Figures

Formulation

IFIH1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

IFIH1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

Hlcd antibody, IDDM19 antibody, MDA-5 antibody, MDA5 antibody, MGC133047 antibody

Database links

Human UniProt/Swiss-Prot:Q9BYX4

Protein names

Hlcd, IDDM19, MDA-5, MDA5, MGC133047

Protein function

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2′-O-methylation at their 5′ cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.

Protein tissue specificity

Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.

Protein sequence and domain

Belongs to the helicase family. RLR subfamily.; Contains 2 CARD domains.; Contains 1 helicase ATP-binding domain.; Contains 1 helicase C-terminal domain.; Contains 1 RLR CTR (RLR C-terminal regulatory) domain.

Protein post-translational modifications

During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation (By similarity).; Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta.; Ubiquitinated. USP17/UPS17L2-dependent deubiquitination positively regulates the receptor.

Protein cellular localization

Cytoplasm . Nucleus . Note: May be found in the nucleus, during apoptosis.

Background

Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4,5). Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5′-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-kappaB, TBK1/IKKepsilon, and IRF-3/IRF-7 (12-15).

Research area

All research areas>Transcription Regulators>MDA5
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize IFIH1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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