Rabbit polyclonal IKBKE antibody (A0244)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal IKBKE antibody (A0244)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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IKBKE antibody,IKK-E antibody,IKK-i antibody,IKKE antibody,IKKI antibody,KIAA0151 antibody,MGC125294 antibody,MGC125295 antibody,MGC125297 antibody
I kappa B kinase epsilon antibody|IKK-E antibody|IKK-epsilon antibody|IKK-i antibody|IKKE antibody|IKKE_HUMAN antibody|IKKepsilon antibody|IKKI antibody|Inducible I kappa B kinase antibody|Inducible I kappa-B kinase antibody|Inducible IkappaB kinase antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody|Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody|KIAA0151 antibody|MGC125294 antibody|MGC125295 antibody|MGC125297 antibody|Anti-IKKi/IKKe antibody (ab7891)

SCBT cat No: sc-101430|sc-52931|sc-376114|sc-365830|sc-374546|sc-10760|

Name

Rabbit polyclonal IKBKE antibody

Catalogue No.

A0244

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human IKBKE

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 80kDa/Observed: Refer to Figures

Formulation

IKBKE antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

IKBKE antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

IKBKE antibody,IKK-E antibody,IKK-i antibody,IKKE antibody,IKKI antibody,KIAA0151 antibody,MGC125294 antibody,MGC125295 antibody,MGC125297 antibody

Database links

Human UniProt/Swiss-Prot:Q14164

Protein names

IKBKE,IKK-E,IKK-i,IKKE,IKKI,KIAA0151,MGC125294,MGC125295,MGC125297

Protein function

Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at ‘Ser-708’. Phosphorylation of STAT1 at ‘Ser-708’ seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.

Protein tissue specificity

Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.

Protein sequence and domain

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Autophosphorylated and phosphorylated by IKBKB/IKKB. Phosphorylation at Ser-172 is enhanced by the interaction with DDX3X. Phosphorylated at Thr-501 upon IFN activation.; Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.; ‘Lys-63’-linked polyubiquitinated at Lys-30 and Lys-401 by TRAF2:BIRC2 and TRAF2:BIRC3 complexes. Ubiquitination is induced by LPS, TNFA and interleukin-1 and required for full kinase activity and KF-kappa-B pathway activation.

Protein cellular localization

Cytoplasm . Nucleus. Nucleus, PML body . Note: Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent . Located diffusely throughout the cytoplasm but locates to punctate cytoplasmic bodies when coexpressed with TRIM6 .

Background

IKK epsilon is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. It has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (1). IKK epsilon phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. It plays a special role in the immune response. IKK epsilon protects cells against DNA damage-induced cell death (2).

Research area

All research areas>Kinases and Phosphatases>IKK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize IKBKE antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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