Rabbit polyclonal IKBKG antibody (A0917)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal IKBKG antibody (A0917)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:100 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal IKBKG antibody.

Alternative names:

Check alternative names for the antibody

Expand

IKBKG antibody,AMCBX1 antibody,FIP-3 antibody,FIP3 antibody,Fip3p antibody,IKK-gamma antibody,IP antibody,IP1 antibody,IP2 antibody,IPD2 antibody,NEMO antibody, IKKgamma antibody
IkB kinase associated protein 1 antibody| IkB kinase subunit gamma antibody| Inhibitor of nuclear factor kappa B kinase subunit gamma antibody|AMCBX1 antibody|FIP 3 antibody|FIP-3 antibody|FIP3 antibody|Fip3p antibody|I kappa B kinase gamma antibody|I-kappa-B kinase subunit gamma antibody|IkB kinase gamma subunit antibody|IkB kinase subunit gamma antibody|IkB kinase-associated protein 1 antibody|Ikbkg antibody|IKK-gamma antibody|IKKAP1 antibody|IKKG antibody|IMD33 antibody|Incontinentia pigmenti antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase gamma antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, gamma antibody|Inhibitor of nuclear factor kappa-B kinase subunit gamma antibody|IP antibody|IP1 antibody|IP2 antibody|IPD2 antibody|NEMO antibody|NEMO_HUMAN antibody|NF kappa B essential modifier antibody|NF kappa B essential modulator antibody|NF-kappa-B essential modifier antibody|NF-kappa-B essential modulator antibody|ZC2HC9 antibody|Anti-IKK gamma antibody [EPR16629] (ab178872)

SCBT cat No: sc-71331|sc-52930|sc-56919|sc-166700|sc-8032|sc-166398|sc-166397|sc-8330|sc-8256|sc-101430|sc-52931|

Name

Rabbit polyclonal IKBKG antibody

Catalogue No.

A0917

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human IKBKG

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:100
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 48kDa/Observed: Refer to Figures

Formulation

IKBKG antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

IKBKG antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

IKBKG antibody,AMCBX1 antibody,FIP-3 antibody,FIP3 antibody,Fip3p antibody,IKK-gamma antibody,IP antibody,IP1 antibody,IP2 antibody,IPD2 antibody,NEMO antibody, IKKgamma antibody

Database links

Human UniProt/Swiss-Prot:Q9Y6K9

Protein names

IKBKG,AMCBX1,FIP-3,FIP3,Fip3p,IKK-gamma,IP,IP1,IP2,IPD2,NEMO, IKKgamma

Protein function

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either ‘Lys-63’-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires ‘Lys-27’-linked polyubiquitination.

Protein tissue specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Protein sequence and domain

The leucine-zipper domain and the CCHC NOA-type zinc-finger are essential for polyubiquitin binding and for the activation of IRF3.Contains 1 CCHC NOA-type zinc finger.

Protein post-translational modifications

Phosphorylation at Ser-68 attenuates aminoterminal homodimerization.; Polyubiquitinated on Lys-285 through ‘Lys-63’; the ubiquitination is mediated by NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-399 through ‘Lys-63’; the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-277 and Lys-309; promotes nuclear export. Polyubiquitinated through ‘Lys-27’ by TRIM23; involved in antiviral innate and inflammatory responses. Linear polyubiquitinated on Lys-111, Lys-143, Lys-226, Lys-246, Lys-264, Lys-277, Lys-285, Lys-292, Lys-302, Lys-309 and Lys-326; the head-to-tail polyubiquitination is mediated by the LUBAC complex and plays a key role in NF-kappa-B activation. Polyubiquitinated on Lys-309 and Lys-321 via ‘Lys-27’-linked ubiquitin by Shigella flexneri E3 ubiquitin-protein ligase ipah9.8, leading to its degradation by the proteasome. Deubiquitinated by USP10 in a TANK-dependent and -independent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage .; Sumoylated on Lys-277 and Lys-309 with SUMO1; the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues.; Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity.

Protein cellular localization

Cytoplasm . Nucleus . Note: Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.

Background

The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins (1-3). Most agents that activate NF-kappaB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkappaB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKalpha and IKKbeta serve as the catalytic subunits of the kinase and IKKgamma serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation of Ser177 and Ser181 in the activation loop of IKKbeta (Ser176 and Ser180 in IKKalpha), which causes conformational changes resulting in kinase activation (10-13).

Research area

All research areas>Kinases and Phosphatases>IKK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize IKBKG antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!