Rabbit Polyclonal IL-1beta antibody (STJ96566)

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Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal IL-1beta antibody (STJ96566)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for IL-1beta Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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IL1B antibody, IL1F2 antibody,|Catabolin antibody|H1 antibody|IL 1 antibody|IL 1 beta antibody|IL-1 beta antibody|IL1 BETA antibody|IL1B antibody|IL1B_HUMAN antibody|IL1F2 antibody|Interleukin 1 beta antibody|Interleukin-1 beta antibody|OAF antibody|OTTHUMP00000162031 antibody|Preinterleukin 1 beta antibody|Pro interleukin 1 beta antibody|Anti-IL1 beta antibody (ab2105)
SCBT cat No: sc-23459|sc-23460|sc-25105|sc-71525|sc-52936|sc-23461|sc-56196|sc-56217|sc-52953|

Name

IL-1beta Polyclonal Antibody

Catalogue No.

STJ96566

Reactivity

Human, Mouse, Rat

Specificity

IL-1beta Polyclonal Antibody detects endogenous levels of IL-1beta protein.

Immunogen

Synthesized peptide derived from IL-1beta at AA range INTERNAL

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

17/30 kDa

Formulation

IL-1beta Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

IL-1beta Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Interleukin-1 beta antibody, IL-1 beta antibody, Catabolin antibody

Database links

Human UniProt/Swiss-Prot:P01584;Mouse UniPort/Swiss-Prot: P10749;Rat UniProt/Swiss-Port: Q5BKB0;Human Entrez Gene: 3553;Mouse Entrez Gene: 16176;Rat Entrez Gene: Rn.9869

Protein names

Interleukin-1 beta , IL-1 beta , Catabolin

Protein function

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

Protein tissue specificity

Expressed in activated monocytes/macrophages (at protein level).

Protein sequence and domain

Belongs to the IL-1 family.

Protein post-translational modifications

Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Protein cellular localization

Cytoplasm > cytosol / Lysosome / Secreted > exosome / Cytoplasmic vesicle > autophagosome / Secreted

Research area

All research areas>Growth Factors and Hormones>IL-1 beta
(View all antibody categories related to Growth Factors and Hormones)

Note

AntibodyPlus can customize IL-1beta Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 27

Validation data ID: 27

Submission date: 2016-11-29

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Result figure:
Result figure

Figure description:

Rat thyrocytes were treated with TSH receptor antibody for 24 hrs., fixed, permeabilized and then stained with primary antibodies. After washing FAR red conjugated secondary antibody was used and then analyzed by LI-COR imaging.

Product name

Beclin-1, MAP LC3beta, LC3A, IL-6, IL-1beta

Product catalog No.

STJ97761, STJ97398, STJ97755, STJ96713, STJ96566

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Good

Your comments

Mouse Monoclonal Beclin-1 antibody [5C2] (STJ97761)
Rabbit Polyclonal MAP LC3beta antibody (STJ97398)
Mouse Monoclonal LC3A antibody [5G10] (STJ97755)
Rabbit Polyclonal IL-6 antibody (STJ96713)
Rabbit Polyclonal IL-1beta antibody (STJ96566)
In fact, I have used them in LI-COR IN CELL WESTERN method. They worked nicely. The method was according to the instructions provided with the LI-COR kit with their buffers and antibodies.

Detailed protocol

View Details

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Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: IL-1beta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: IL-1beta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse heart tissue

1: IL-1beta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: IL-1beta Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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