Rabbit Polyclonal IL-6 antibody (STJ96713)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal IL-6 antibody (STJ96713)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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IL6 antibody, IFNB2 antibody,|Interleukin BSF 2 antibody|B cell differentiation factor antibody|B cell stimulatory factor 2 antibody|B-cell stimulatory factor 2 antibody|BSF 2 antibody|BSF-2 antibody|BSF2 antibody|CDF antibody|CTL differentiation factor antibody|Hepatocyte stimulatory factor antibody|HGF antibody|HSF antibody|Hybridoma growth factor antibody|Hybridoma growth factor Interferon beta-2 antibody|IFN-beta-2 antibody|IFNB2 antibody|IL 6 antibody|IL-6 antibody|IL6 antibody|IL6_HUMAN antibody|Interferon beta 2 antibody|Interferon beta-2 antibody|Interleukin 6 antibody|Interleukin 6 (interferon beta 2) antibody|Interleukin BSF 2 antibody|Interleukin-6 antibody|Anti-IL6 antibody (ab6672)
SCBT cat No: sc-130326|sc-65989|sc-53865|sc-65327|sc-32296|sc-323975|sc-28343|sc-7920|sc-385224|

Name

IL-6 Polyclonal Antibody

Catalogue No.

STJ96713

Reactivity

Human

Specificity

IL-6 Polyclonal Antibody detects endogenous levels of IL-6 protein.

Immunogen

Synthesized peptide derived from IL-6 at AA range INTERNAL

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

25 kDa

Formulation

IL-6 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

IL-6 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Interleukin-6 antibody, IL-6 antibody, B-cell stimulatory factor 2 antibody, BSF-2 antibody, CTL differentiation factor antibody, CDF antibody, Hybridoma growth factor antibody, Interferon beta-2 antibody, IFN-beta-2 antibody

Database links

Human UniProt/Swiss-Prot:P05231;Mouse UniPort/Swiss-Prot: P08505;Rat UniProt/Swiss-Port: P20607;Human Entrez Gene: 3569;Mouse Entrez Gene: 16193;Rat Entrez Gene: Rn.9873

Protein names

Interleukin-6 , IL-6 , B-cell stimulatory factor 2 , BSF-2 , CTL differentiation factor , CDF , Hybridoma growth factor , Interferon beta-2 , IFN-beta-2

Protein function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

Involvement in disease

Rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]: An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. . Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.; Note: A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. .

Protein sequence and domain

Belongs to the IL-6 superfamily.

Protein post-translational modifications

N- and O-glycosylated.

Protein cellular localization

Secreted

Research area

All research areas>Growth Factors and Hormones>IL-6
(View all antibody categories related to Growth Factors and Hormones)

Note

AntibodyPlus can customize IL-6 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 27

Validation data ID: 27

Submission date: 2016-11-29

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Result figure:
Result figure

Figure description:

Rat thyrocytes were treated with TSH receptor antibody for 24 hrs., fixed, permeabilized and then stained with primary antibodies. After washing FAR red conjugated secondary antibody was used and then analyzed by LI-COR imaging.

Product name

Beclin-1, MAP LC3beta, LC3A, IL-6, IL-1beta

Product catalog No.

STJ97761, STJ97398, STJ97755, STJ96713, STJ96566

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Good

Your comments

Mouse Monoclonal Beclin-1 antibody [5C2] (STJ97761)
Rabbit Polyclonal MAP LC3beta antibody (STJ97398)
Mouse Monoclonal LC3A antibody [5G10] (STJ97755)
Rabbit Polyclonal IL-6 antibody (STJ96713)
Rabbit Polyclonal IL-1beta antibody (STJ96566)
In fact, I have used them in LI-COR IN CELL WESTERN method. They worked nicely. The method was according to the instructions provided with the LI-COR kit with their buffers and antibodies.

Detailed protocol

View Details

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Immunohistochemical analysis of paraffin embedded Human breast cancer tissue

1: IL-6 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: IL-6 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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