Rabbit Polyclonal IL8 antibody (STJ96518)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal IL8 antibody (STJ96518)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CXCL8 antibody, IL8 antibody,|(Ala-IL-8)77 antibody|(Ser-IL-8)72 antibody|9E3 antibody|Beta thromboglobulin like protein antibody|C-X-C motif chemokine 8 antibody|CEF-4 antibody|chemokine, CXC motif, ligand 8 antibody|CXCL8 antibody|Emoctakin antibody|GCP-1 antibody|GCP/IL-8 protein I antibody|GCP/IL-8 protein II antibody|GCP/IL-8 protein III antibody|GCP/IL-8 protein IV antibody|GCP/IL-8 protein V antibody|GCP/IL-8 protein VI antibody|GCP1 antibody|Granulocyte chemotactic protein 1 antibody|IL-8 antibody|IL-8(1-77) antibody|IL-8(9-77) antibody|IL8 antibody|IL8/NAP1 form I antibody|IL8/NAP1 form II antibody|IL8/NAP1 form III antibody|IL8/NAP1 form IV antibody|IL8/NAP1 form V antibody|IL8/NAP1 form VI antibody|IL8_HUMAN antibody|Inteleukin 8 antibody|LECT antibody|LUCT antibody|Lymphocyte-derived neutrophil-activating factor antibody|LYNAP antibody|MDNCF antibody|MDNCF-b antibody|MDNCF-c antibody|MONAP antibody|Monocyte-derived neutrophil chemotactic factor antibody|Monocyte-derived neutrophil-activating peptide antibody|NAF antibody|NAP-1 antibody|NAP1 antibody|Neutrophil activating peptide 1 antibody|Neutrophil activating protein 1 antibody|Neutrophil-activating factor antibody|Neutrophil-activating protein 1 antibody|Protein 3 10C antibody|Protein 3-10C antibody|SCYB8 antibody|Small inducible cytokine subfamily B member 8 antibody|T cell chemotactic factor antibody|T-cell chemotactic factor antibody|TSG1 antibody|Anti-IL8 antibody (ab7747)
SCBT cat No: sc-32817|sc-101499|sc-52870|sc-7302|sc-8427|sc-376750|sc-1269|sc-7922|sc-73321|

Name

IL-8 Polyclonal Antibody

Catalogue No.

STJ96518

Reactivity

Human

Specificity

IL-8 Polyclonal Antibody detects endogenous levels of IL-8 protein.

Immunogen

Synthesized peptide derived from IL-8 at AA range C-TERM

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

11 kDa

Formulation

IL-8 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

IL-8 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Interleukin-8 antibody, IL-8 antibody, C-X-C motif chemokine 8 antibody, Chemokine antibody, C-X-C motif antibody, ligand 8 antibody, Emoctakin antibody, Granulocyte chemotactic protein 1 antibody, GCP-1 antibody, Monocyte-derived neutrophil chemotactic factor antibody, MDNCF antibody, Monocyte-derived neutrophil-activating peptide antibody, MONAP antibody, Neutrophil-activating protein 1 antibody, NAP-1 antibody, Protein 3-10C antibody, T-cell chemotactic factor antibody

Database links

Human UniProt/Swiss-Prot:P10145;Human Entrez Gene: 3576;

Protein names

Interleukin-8 , IL-8 , C-X-C motif chemokine 8 , Chemokine , C-X-C motif , ligand 8 , Emoctakin , Granulocyte chemotactic protein 1 , GCP-1 , Monocyte-derived neutrophil chemotactic factor , MDNCF , Monocyte-derived neutrophil-activating peptide , MONAP , Neutrophil-activating protein 1 , NAP-1 , Protein 3-10C , T-cell chemotactic factor

Protein function

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

Protein tissue specificity

By ER stress in a DDIT3/CHOP-dependent manner.

Protein sequence and domain

Belongs to the intercrine alpha (chemokine CxC) family.

Protein post-translational modifications

Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. / Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.

Protein cellular localization

Secreted

Research area

All research areas>Growth Factors and Hormones>IL-8
(View all antibody categories related to Growth Factors and Hormones)

Note

AntibodyPlus can customize IL-8 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast cancer tissue

1: IL-8 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: IL-8 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: IL-8 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: IL-8 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human kidney tissue

1: IL-8 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human kidney tissue

1: IL-8 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: IL-8 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: IL-8 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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