Rabbit polyclonal INCENP antibody (A0622)

$69.00$259.00

Reactivity: Human,Rat
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal INCENP antibody (A0622)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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binds and activates aurora B and C in vivo and in vitro antibody|Chromosomal passenger protein antibody|INCE_HUMAN antibody|INCENP antibody|Inner centromere protein antibody|Inner centromere protein antigens 135/155kDa antibody|Inner centromere protein antigens 135kD 155kD antibody|Inner centromere protein INCENP antibody|Anti-INCENP antibody (ab12183)

SCBT cat No: sc-376514|sc-47894|sc-47895|

Name

Rabbit polyclonal INCENP antibody

Catalogue No.

A0622

Reactivity

Human, Rat

Immunogen

Recombinant protein of human INCENP

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 140kDa/Observed: Refer to Figures

Formulation

INCENP antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

INCENP antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Database links

Human UniProt/Swiss-Prot:Q9NQS7

Protein function

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.

Protein sequence and domain

Belongs to the INCENP family.

Protein post-translational modifications

Phosphorylation by AURKB at its C-terminal part is important for AURKB activation by INCENP.

Protein cellular localization

Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Midbody . Chromosome, centromere, kinetochore . Note: Colocalized at synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocenters. Colocalizes with AURKB at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone (By similarity). Colocalizes with AURKB at mitotic chromosomes . Localizes to inner kinetochore . Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis . Cocalizes to the equatorial cell cortex at anaphase .

Background

In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and ‘passenger,’ or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term ‘passenger proteins’ encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis

Note

AntibodyPlus can customize INCENP antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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