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Rabbit polyclonal IRF3 antibody (A2172)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF,IP,ChIP
WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:50 – 1:200 ChIP 1:20 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal IRF3 antibody.
Check alternative names for the antibodyExpand
Interferon regulatory factor 3 antibody|IRF 3 antibody|IRF-3 antibody|IRF3 antibody|IRF3_HUMAN antibody|MGC94729 antibody|Anti-IRF3 antibody [EPR2418Y] (ab68481)
SCBT cat No: sc-15991|sc-376455|sc-9082|sc-33641|sc-33642|
Rabbit polyclonal IRF3 antibody
|Catalogue No.|| |
Human, Mouse, Rat
A synthetic Peptide of human IRF3
WB, IHC, IF, IP, ChIP
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 427aa/Observed: Refer to figures
IRF3 antibody was tube-contained.
IRF3 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Database links|| |
|Protein function|| |
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
|Protein tissue specificity|| |
Expressed constitutively in a variety of tissues.
|Protein sequence and domain|| |
Belongs to the IRF family.; Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
|Protein post-translational modifications|| |
Constitutively phosphorylated on many Ser/Thr residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Phosphorylated at Ser-396 by IKBKE upon ssRNA viral infection. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. Phosphorylation at Ser-386 by TBK1 results in oligomerization. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection.; (Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.; Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.; ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
|Protein cellular localization|| |
Cytoplasm . Nucleus . Note: Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
AntibodyPlus can customize IRF3 antibody according to your requirement, including bulk product size,etc. Please contact firstname.lastname@example.org. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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