Rabbit polyclonal IRF3 antibody (A2172)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF,IP,ChIP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal IRF3 antibody (A2172)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF,IP,ChIP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:50 – 1:200 ChIP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal IRF3 antibody.

Alternative names:

Check alternative names for the antibody

Expand

Interferon regulatory factor 3 antibody|IRF 3 antibody|IRF-3 antibody|IRF3 antibody|IRF3_HUMAN antibody|MGC94729 antibody|Anti-IRF3 antibody [EPR2418Y] (ab68481)

SCBT cat No: sc-15991|sc-376455|sc-9082|sc-33641|sc-33642|

Name

Rabbit polyclonal IRF3 antibody

Catalogue No.

A2172

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic Peptide of human IRF3

Host

Rabbit

Applications

WB, IHC, IF, IP, ChIP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:50 – 1:200
ChIP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 427aa/Observed: Refer to figures

Formulation

IRF3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

IRF3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Database links

Human UniProt/Swiss-Prot:Q14653

Protein function

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Protein tissue specificity

Expressed constitutively in a variety of tissues.

Protein sequence and domain

Belongs to the IRF family.; Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Protein post-translational modifications

Constitutively phosphorylated on many Ser/Thr residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Phosphorylated at Ser-396 by IKBKE upon ssRNA viral infection. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. Phosphorylation at Ser-386 by TBK1 results in oligomerization. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection.; (Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.; Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.; ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.

Protein cellular localization

Cytoplasm . Nucleus . Note: Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.

Research area

All research areas>Transcription Regulators>IRF
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize IRF3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!