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Rabbit Polyclonal JAK1 antibody (STJ93789)
$99.00–$319.00
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.
Rabbit Polyclonal JAK1 antibody (STJ93789)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
Recommended protocols: check protocols
Image descriptions:
Click or hover above images to see image description for JAK1 Polyclonal Antibody.
Alternative names:
Check alternative names for the antibody
ExpandJAK1 antibody, JAK1A antibody, JAK1B antibody,|JAK 1 antibody|JAK 1A antibody|JAK 1B antibody|JAK-1 antibody|JAK1 antibody|JAK1_HUMAN antibody|JAK1A antibody|JAK1B antibody|Janus kinase 1 (a protein tyrosine kinase) antibody|Janus kinase 1 antibody|JTK3 antibody|Tyrosine protein kinase JAK 1 antibody|Tyrosine protein kinase JAK1 antibody|Tyrosine-protein kinase JAK1 antibody|Phospho anti-JAK1 (Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
SCBT cat No: sc-136225|sc-1677|sc-376996|sc-7228|sc-277|sc-295|sc-390539|sc-34479|
| Name | JAK1 Polyclonal Antibody |
|---|---|
| Catalogue No. | STJ93789 |
| Reactivity | Human, Mouse, Rat |
| Specificity | JAK1 Polyclonal Antibody detects endogenous levels of JAK1 protein. |
| Immunogen | Synthesized peptide derived from JAK1 at AA range 960-1040 |
| Host | Rabbit |
| Applications | WB, IHC, ELISA |
| Recommended dilution | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Molecular weight | 130 kDa |
| Formulation | JAK1 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Concentration | 1 mg/ml |
| Purification | JAK1 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -20 Celsius degree. Avoid repeated freeze/thaw cycles. |
| Alternative antibody names | Tyrosine-protein kinase JAK1 antibody, Janus kinase 1 antibody, JAK-1 antibody |
| Database links | Human UniProt/Swiss-Prot:P23458;Rat UniProt/Swiss-Port: G3V9W2;Human Entrez Gene: 3716;Mouse Entrez Gene: 16451;Rat Entrez Gene: Rn.90191 |
| Protein names | Tyrosine-protein kinase JAK1 , Janus kinase 1 , JAK-1 |
| Protein function | Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway . Kinase partner for the interleukin (IL)-2 receptor . / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Mg2+ |
| Protein tissue specificity | Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue. |
| Protein sequence and domain | Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1. / The FERM domain mediates interaction with JAKMIP1. / Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. / Contains 1 FERM domain. / Contains 2 protein kinase domains. / Contains 1 SH2 domain. |
| Protein post-translational modifications | Autophosphorylated . Phosphorylated on tyrosine residues in response to interferon gamma signaling . Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling . |
| Protein cellular localization | Endomembrane system; Peripheral membrane protein |
| Research area | All research areas>Kinases and Phosphatases>JAK |
| Note | AntibodyPlus can customize JAK1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews. |
| Supplier | St John’s Laboratory Ltd. |
| Product type | Primary antibody |
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Immunofluorescence analysis of Rat lung tissue
1: JAK1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
Immunofluorescence analysis of Rat lung tissue
1: JAK1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
Immunofluorescence analysis of Rat kidney tissue
1: JAK1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
Immunofluorescence analysis of Rat spleen tissue
1: JAK1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
Immunofluorescence analysis of Rat spleen tissue
1: JAK1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
Immunohistochemical analysis of paraffin embedded Human uterus tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Human Tonsil tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Human liver tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Human liver cancer tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Human lung tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Human lung cancer tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Human stomach tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Rat heart tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Rat testis tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Rat liver tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Rat lung tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Rat kidney tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Rat spinal cord tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Rat brain tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Rat spleen tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Mouse heart tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Mouse testis tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Mouse liver tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Mouse lung tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Mouse kidney tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin embedded Mouse spleen tissue
1: JAK1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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