Rabbit Polyclonal Ki67 antibody (STJ93832)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Ki67 antibody (STJ93832)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC, IF, ELISA

Recommended dilution: IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Ki-67 Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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MKI67 antibody,|Antigen identified by monoclonal antibody Ki 67 antibody|Protein phosphatase 1 regulatory subunit 105 antibody|RP11-380J17.2 antibody|Anti-Ki67 antibody (ab15580)
SCBT cat No: sc-7844|sc-15402|sc-23900|sc-7846|sc-56319|sc-101861|

Name

Ki-67 Polyclonal Antibody

Catalogue No.

STJ93832

Reactivity

Human

Specificity

Ki-67 Polyclonal Antibody detects endogenous levels of Ki-67 protein.

Immunogen

Synthesized peptide derived from Ki-67 at AA range 3170-3250

Host

Rabbit

Applications

IHC, IF, ELISA

Recommended dilution

IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

358.694 kDa

Formulation

Ki-67 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Ki-67 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Proliferation marker protein Ki-67 antibody, Antigen identified by monoclonal antibody Ki-67 antibody, Antigen KI-67 antibody, Antigen Ki67 antibody

Database links

Human UniProt/Swiss-Prot:P46013;Rat UniProt/Swiss-Port: D4A0Y6;Human Entrez Gene: 4288;Mouse Entrez Gene: 17345;Rat Entrez Gene: Rn.233802

Protein names

Proliferation marker protein Ki-67 , Antigen identified by monoclonal Ki-67 , Antigen KI-67 , Antigen Ki67

Protein function

Thought to be required for maintaining cell proliferation.

Protein tissue specificity

Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.

Protein sequence and domain

Contains 1 FHA domain.

Protein cellular localization

Nucleus / Nucleus > nucleolus / Chromosome

Research area

All research areas>Cell Cycle Proteins>Ki-67
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize Ki-67 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast cancer tissue

1: Ki-67 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: Ki-67 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: Ki-67 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: Ki-67 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: Ki-67 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: Ki-67 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human lung tissue

1: Ki-67 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: Ki-67 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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