Rabbit polyclonal KPNA2 antibody (A1623)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal KPNA2 antibody (A1623)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal KPNA2 antibody.

Alternative names:

Check alternative names for the antibody

Expand

QIP2 antibody, RCH1 antibody, IPOA1 antibody, SRP1alpha antibody
IMA1_HUMAN antibody|Importin alpha 1 antibody|Importin alpha 2 antibody|Importin alpha 2 subunit antibody|Importin alpha P1 antibody|Importin subunit alpha-1 antibody|IPO A1 antibody|IPOA 1 antibody|IPOA1 antibody|Karyopherin alpha 2 antibody|Karyopherin alpha 2 RAG cohort 1 antibody|Karyopherin alpha 2 subunit antibody|Karyopherin subunit alpha-2 antibody|KPNA2 antibody|KPNA2 protein antibody|Pendulin antibody|QIP 2 antibody|QIP2 antibody|RAG cohort 1 antibody|RAG cohort protein 1 antibody|RCH 1 antibody|RCH1 antibody|SRP 1 antibody|SRP1 alpha antibody|SRP1 antibody|SRP1-alpha antibody|SRP1alpha antibody|Anti-KPNA2 antibody (ab84440)

SCBT cat No: sc-101538|sc-57009|sc-136204|sc-55538|sc-6917|sc-55537|sc-514663|

Name

Rabbit polyclonal KPNA2 antibody

Catalogue No.

A1623

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human KPNA2

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 58kDa/Observed: Refer to Figures

Formulation

KPNA2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

KPNA2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

QIP2 antibody, RCH1 antibody, IPOA1 antibody, SRP1alpha antibody

Database links

Human UniProt/Swiss-Prot:P52292

Protein names

QIP2, RCH1, IPOA1, SRP1alpha

Protein function

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Protein tissue specificity

Expressed ubiquitously.

Protein sequence and domain

Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.; The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins (By similarity).; The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding (By similarity).Belongs to the importin alpha family.; Contains 10 ARM repeats.; Contains 1 IBB domain.

Protein cellular localization

Cytoplasm . Nucleus .

Background

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008]

Research area

All research areas>Transport and Trafficking>karyopherin
(View all antibody categories related to Transport and Trafficking)

Note

AntibodyPlus can customize KPNA2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!