Rabbit polyclonal LEP antibody (A1300)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal LEP antibody (A1300)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:200 – 1:500 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

LEP antibody, OB antibody, OBS antibody, Leptin antibody, Obese protein antibody, Obesity factor antibody
FLJ94114 antibody|LEP antibody|LEP_HUMAN antibody|LEPD antibody|Leptin (murine obesity homolog) antibody|Leptin (obesity homolog, mouse) antibody|Leptin antibody|Leptin Murine Obesity Homolog antibody|Leptin Precursor Obesity Factor antibody|OB antibody|Obese protein antibody|Obese, mouse, homolog of antibody|Obesity antibody|Obesity factor antibody|Obesity homolog mouse antibody|Obesity Murine Homolog Leptin antibody|OBS antibody|OTTHUMP00000212285 antibody|Anti-Leptin antibody (ab3583)

SCBT cat No: sc-842|sc-28344|sc-48408|sc-9014|sc-393043|sc-843|

Name

Rabbit polyclonal LEP antibody

Catalogue No.

A1300

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human LEP

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:200 – 1:500
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 19kDa/Observed: Refer to Figures

Formulation

LEP antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

LEP antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

LEP antibody, OB antibody, OBS antibody, Leptin antibody, Obese protein antibody, Obesity factor antibody

Database links

Human UniProt/Swiss-Prot:P41159

Protein names

LEP, OB, OBS, Leptin, Obese protein, Obesity factor

Protein function

Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways . In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity) . In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption . Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity) . May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression . Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) . In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells . Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 . In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation .

Protein tissue specificity

Adipose tissue is the main source of leptin it is also produced by other peripheral tissues such as the skeletal muscle . Expressed by intercalated and striated tracts of submandibular and parotid salivary gland intralobular ducts . Detected by fundic epithelium of the gastric mucosa . Secreted into blood and gastric juice .

Protein sequence and domain

Belongs to the leptin family.

Protein cellular localization

Secreted .

Background

Leptin is a 16 kDa adipocyte-derived hormone that relays the status of the body’s energy reserves to the hypothalamus, resulting in the suppression of appetite and modulation of energy expenditure (1,2). Leptin functions as a pleiotropic cytokine with multiple roles in immune function and reproduction (1-4). There are at least five isoforms of the leptin receptor, but only OB-Rb is signaling competent (1,2). OB-Rb is expressed on a number of cells, including T cells, B cells, keratinocytes, and others (1-6). Leptin/OB-Rb interactions induce Jak2, PI3K, Erk1/2, and Stat3 activation (1,2). OB-Rb is often highly expressed in many tumor types, including breast, pancreatic, and Tumor Initiating Stem Cells (TISCS), providing a potential mechanistic link for the tumor promoting effects of obesity (2,7).

Research area

All research areas>Growth Factors and Hormones>Ob
(View all antibody categories related to Growth Factors and Hormones)

Note

AntibodyPlus can customize LEP antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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