Rabbit polyclonal LEPR antibody (A2999)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal LEPR antibody (A2999)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:1000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal LEPR antibody.

Alternative names:

Check alternative names for the antibody

Expand

OBR antibody, OB-R antibody, CD295 antibody, LEP-R antibody
CD 295 antibody|Leptin receptor precursor antibody|OB receptor antibody|OB-R antibody|OB-RGRP antibody|obl antibody|Obr antibody|Anti-Leptin Receptor antibody (ab5593)

SCBT cat No: sc-1835|sc-1834|sc-1833|

Name

Rabbit polyclonal LEPR antibody

Catalogue No.

A2999

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human LEPR

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:1000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 96kDa/Observed: Refer to Figures

Formulation

LEPR antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

LEPR antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

OBR antibody, OB-R antibody, CD295 antibody, LEP-R antibody

Database links

Human UniProt/Swiss-Prot:P48357

Protein names

OBR, OB-R, CD295, LEP-R

Protein function

Receptor for hormone LEP/leptin (Probable) . On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (By similarity) . In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity . Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity).; Isoform A: May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3.; Isoform E: Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.

Protein tissue specificity

Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus, but also in skeletal muscle. Detected in fundic and antral epithelial cells of the gastric mucosa . Isoform B and isoform A are expressed by NK cells (at protein level) .

Protein sequence and domain

The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.; The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.; The box 1 motif is required for JAK interaction and/or activation.Belongs to the type I cytokine receptor family. Type 2 subfamily.; Contains 4 fibronectin type-III domains.; Contains 1 Ig-like (immunoglobulin-like) domain.

Protein post-translational modifications

On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role.

Protein cellular localization

Cell membrane ; Single-pass type I membrane protein . Basolateral cell membrane .; Isoform E: Secreted .

Background

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).

Research area

All research areas>Membrane Receptors>Ob-R
(View all antibody categories related to Membrane Receptors)

Note

AntibodyPlus can customize LEPR antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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