Rabbit polyclonal LMNA antibody (A0249)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal LMNA antibody (A0249)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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LMNA antibody,CDCD1 antibody,CDDC antibody,CMD1A antibody,CMT2B1 antibody,EMD2 antibody,FPL antibody,FPLD antibody,HGPS antibody,IDC antibody,LDP1 antibody,LFP antibody,LGMD1B antibody,LMN1 antibody,LMNC antibody,PRO1 antibody
70 kDa lamin antibody|CDDC antibody|EMD2 antibody|FPL antibody|FPLD antibody|HGPS antibody|IDC antibody|LAMIN A antibody|lamin A/C antibody|LAMIN C antibody|Lamin-A/C antibody|LDP1 antibody|LFP antibody|LMN 1 antibody|LMN A antibody|LMN C antibody|LMNA antibody|LMNA_HUMAN antibody|LMNC antibody|PRO1 antibody|Renal carcinoma antigen NY-REN-32 antibody|Anti-Lamin A + C antibody [131C3] (ab8984)

SCBT cat No: sc-25105|sc-71525|sc-52936|sc-23461|sc-56196|sc-56217|sc-52953|sc-71965|sc-23465|sc-23464|sc-23335|sc-73850|sc-49413|sc-49414|sc-393134|

Name

Rabbit polyclonal LMNA antibody

Catalogue No.

A0249

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human LMNA

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 65kDa/Observed: Refer to Figures

Formulation

LMNA antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

LMNA antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

LMNA antibody,CDCD1 antibody,CDDC antibody,CMD1A antibody,CMT2B1 antibody,EMD2 antibody,FPL antibody,FPLD antibody,HGPS antibody,IDC antibody,LDP1 antibody,LFP antibody,LGMD1B antibody,LMN1 antibody,LMNC antibody,PRO1 antibody

Database links

Human UniProt/Swiss-Prot:P02545

Protein names

LMNA,CDCD1,CDDC,CMD1A,CMT2B1,EMD2,FPL,FPLD,HGPS,IDC,LDP1,LFP,LGMD1B,LMN1,LMNC,PRO1

Protein function

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone.; Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence.

Protein tissue specificity

In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress.

Protein sequence and domain

Belongs to the intermediate filament family.; Contains 1 LTD domain.

Protein post-translational modifications

Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.; Proteolytic cleavage of the C-terminal of 18 residues of prelamin-A/C results in the production of lamin-A/C. The prelamin-A/C maturation pathway includes farnesylation of CAAX motif, ZMPSTE24/FACE1 mediated cleavage of the last three amino acids, methylation of the C-terminal cysteine and endoproteolytic removal of the last 15 C-terminal amino acids. Proteolytic cleavage requires prior farnesylation and methylation, and absence of these blocks cleavage.; Sumoylation is necessary for the localization to the nuclear envelope.; Farnesylation of prelamin-A/C facilitates nuclear envelope targeting.

Protein cellular localization

Nucleus. Nucleus envelope. Nucleus lamina. Nucleus, nucleoplasm. Note: Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C.; Isoform C: Nucleus speckle .

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

Research area

All research areas>Tumor Suppressor/Apoptosis>Lamin
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize LMNA antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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