Rabbit Polyclonal MAP LC3beta antibody (STJ97398)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Antibody Validation Project, Antibody Trial Sample, Antibody Free Sample for your own antibody validation and submitting antibody reviews, AntibodyPlus

Rabbit Polyclonal MAP LC3beta antibody (STJ97398)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:1000-2000; IHC 1:200-500

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for MAP LC3beta Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

MAP1LC3B antibody, MAP1ALC3 antibody,|ATG8F antibody|LC3B antibody|LC3II antibody|MAP1 light chain 3 like protein 2 antibody|MAP1 light chain 3-like protein 2 antibody|MAP1A/1BLC3 antibody|MAP1A/MAP1B LC3 B antibody|MAP1A/MAP1B light chain 3 B antibody|MAP1ALC3 antibody|MAP1LC3B a antibody|Map1lc3b antibody|Microtubule associated protein 1 light chain 3 beta antibody|Microtubule-associated protein 1 light chain 3 beta antibody|Microtubule-associated proteins 1A/1B light chain 3B antibody|MLP3B_HUMAN antibody|Anti-LC3B antibody (ab48394)
SCBT cat No: sc-16756|sc-398822|sc-292354|sc-54237|sc-271625|sc-376404|sc-28266|

Name

MAP LC3beta Polyclonal Antibody

Catalogue No.

STJ97398

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous MAP LC3beta protein.

Immunogen

Recombinant Protein

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:1000-2000; IHC 1:200-500

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Formulation

MAP LC3beta Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

MAP LC3beta Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Microtubule-associated proteins 1A/1B light chain 3B antibody, Autophagy-related protein LC3 B antibody, Autophagy-related ubiquitin-like modifier LC3 B antibody, MAP1 light chain 3-like protein 2 antibody, MAP1A/MAP1B light chain 3 B antibody, MAP1A/MAP1B LC3 B antibody, Microtubule-associated protein 1 light chain 3 beta antibody

Database links

Human UniProt/Swiss-Prot:Q9GZQ8;Mouse UniPort/Swiss-Prot: Q9CQV6;Rat UniProt/Swiss-Port: Q62625;Human Entrez Gene: 81631;Mouse Entrez Gene: 67443;Rat Entrez Gene: Rn.41412

Protein names

Microtubule-associated proteins 1A/1B light chain 3B , Autophagy-related protein LC3 B , Autophagy-related ubiquitin-like modifier LC3 B , MAP1 light chain 3-like protein 2 , MAP1A/MAP1B light chain 3 B , MAP1A/MAP1B LC3 B , Microtubule-associated protein 1 light chain 3 beta

Protein function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Protein tissue specificity

Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.

Protein sequence and domain

Belongs to the ATG8 family.

Protein post-translational modifications

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II . / The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. / Phosphorylation at Thr-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.

Protein cellular localization

Cytoplasm > cytoskeleton / Endomembrane system; Lipid-anchor / Cytoplasmic vesicle > autophagosome membrane; Lipid-anchor / Cytoplasmic vesicle > autophagosome

Research area

All research areas>Structural Proteins>MAP LC3 beta
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize MAP LC3beta Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

Displaying 1 - 1 of 1

Antibody customer reviews & validation data - 27

Validation data ID: 27

Submission date: 2016-11-29

View Details

Result figure:
Result figure

Figure description:

Rat thyrocytes were treated with TSH receptor antibody for 24 hrs., fixed, permeabilized and then stained with primary antibodies. After washing FAR red conjugated secondary antibody was used and then analyzed by LI-COR imaging.

Product name

Beclin-1, MAP LC3beta, LC3A, IL-6, IL-1beta

Product catalog No.

STJ97761, STJ97398, STJ97755, STJ96713, STJ96566

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Good

Your comments

Mouse Monoclonal Beclin-1 antibody [5C2] (STJ97761)
Rabbit Polyclonal MAP LC3beta antibody (STJ97398)
Mouse Monoclonal LC3A antibody [5G10] (STJ97755)
Rabbit Polyclonal IL-6 antibody (STJ96713)
Rabbit Polyclonal IL-1beta antibody (STJ96566)
In fact, I have used them in LI-COR IN CELL WESTERN method. They worked nicely. The method was according to the instructions provided with the LI-COR kit with their buffers and antibodies.

Detailed protocol

View Details

view database submit reviews
 

Immunofluorescence analysis of Mouse lung tissue

1: LC3B Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: LC3B Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: LC3B Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue

1: LC3B Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: LC3B Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: LC3B Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: LC3B Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat brain tissue

1: LC3B Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: LC3B Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


Welcome to AntibodyPlus!