Rabbit polyclonal MAP1LC3A antibody (A5618)

Rabbit polyclonal MAP1LC3A antibody

Human, Mouse, Rat

Rabbit

WB 1:500 – 1:2000
IF 1:50 – 1:200

Unconjugated

Predicted: 14kDa/Observed: Refer to Figures

MAP1LC3A antibody was purified using affinity purification.

LC3 antibody, LC3A antibody, ATG8E antibody, MAP1ALC3 antibody, MAP1BLC3 antibody

LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II .; The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.; Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

Primary antibody