Rabbit polyclonal MAP2K1 antibody (A0252)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal MAP2K1 antibody (A0252)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal MAP2K1 antibody.

Alternative names:

Check alternative names for the antibody

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MAP2K1 antibody,MAPKK1 antibody,MEK1 antibody,MKK1 antibody,PRKMK1 antibody
Dual specificity mitogen activated protein kinase kinase 1 antibody|Dual specificity mitogen-activated protein kinase kinase 1 antibody|ERK activator kinase 1 antibody|MAP kinase kinase 1 antibody|MAP kinase/Erk kinase 1 antibody|MAP2K1 antibody|MAPK/ERK kinase 1 antibody|MAPKK 1 antibody|MAPKK1 antibody|MEK 1 antibody|Mek1 antibody|MEKK1 antibody|Mitogen activated protein kinase kinase 1 antibody|MKK 1 antibody|MKK1 antibody|MP2K1_HUMAN antibody|PRKMK1 antibody|Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody|Protein kinase mitogen activated, kinase 1 antibody|Phospho anti-MEK1 (S298) antibody [EPR3338] (ab96379)

SCBT cat No: sc-365800|sc-219|sc-6250|sc-436|sc-81504|sc-81473|sc-136261|sc-13159|sc-525|sc-524|sc-135985|

Name

Rabbit polyclonal MAP2K1 antibody

Catalogue No.

A0252

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human MAP2K1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 43kDa/Observed: Refer to Figures

Formulation

MAP2K1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

MAP2K1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MAP2K1 antibody,MAPKK1 antibody,MEK1 antibody,MKK1 antibody,PRKMK1 antibody

Database links

Human UniProt/Swiss-Prot:Q02750

Protein names

MAP2K1,MAPKK1,MEK1,MKK1,PRKMK1

Protein function

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Protein tissue specificity

Widely expressed, with extremely low levels in brain.

Protein sequence and domain

The proline-rich region localized between residues 270 and 307 is important for binding to RAF1 and activation of MAP2K1/MEK1.Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK.; Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

Protein cellular localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body . Cytoplasm . Nucleus . Membrane ; Peripheral membrane protein . Note: Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis . Membrane localization is probably regulated by its interaction with KSR1 .

Background

MAP2K1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MAP2K1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MAP2K1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MAP2K1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

Research area

All research areas>Kinases and Phosphatases>MEK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize MAP2K1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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