Rabbit polyclonal MAP2K7 antibody (A2186)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,ICC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal MAP2K7 antibody (A2186)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,ICC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 ICC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal MAP2K7 antibody.

Alternative names:

Check alternative names for the antibody

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MAP2K7 antibody, Jnkk2 antibody, MAPKK7 antibody, MKK7 antibody, PRKMK7 antibody, SAPKK-4 antibody, SAPKK4 antibody
c-Jun N-terminal kinase kinase 2 antibody|Dual specificity mitogen activated protein kinase kinase 7 antibody|Dual specificity mitogen-activated protein kinase kinase 7 antibody|JNK activating kinase 2 antibody|JNK kinase 2 antibody|JNK-activating kinase 2 antibody|JNKK 2 antibody|Jnkk-2 antibody|Jnkk2 antibody|MAP kinase kinase 7 antibody|MAP2K7 antibody|MAPK/ERK kinase 7 antibody|MAPKK 7 antibody|MAPKK-7 antibody|MAPKK7 antibody|MEK 7 antibody|Mitogen Activated Protein Kinase kinase 7 antibody|MKK 7 antibody|MKK-7 antibody|MKK7 antibody|MP2K7_HUMAN antibody|PRKMK 7 antibody|PRKMK-7 antibody|PRKMK7 antibody|SAPK kinase 4 antibody|SAPKK-4 antibody|SAPKK4 antibody|Sek 2 antibody|Sek-2 antibody|Sek2 antibody|SKK4 antibody|stress-activated protein kinase kinase 4 antibody|Anti-MEK7 antibody [EP1455Y] (ab52618) KO Validated

SCBT cat No: sc-7103|sc-25288|sc-13071|sc-7104|

Name

Rabbit polyclonal MAP2K7 antibody

Catalogue No.

A2186

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human MAP2K7

Host

Rabbit

Applications

WB, IHC, ICC, IF, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
ICC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 47kDa/Observed: Refer to Figures

Formulation

MAP2K7 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

MAP2K7 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MAP2K7 antibody, Jnkk2 antibody, MAPKK7 antibody, MKK7 antibody, PRKMK7 antibody, SAPKK-4 antibody, SAPKK4 antibody

Database links

Human UniProt/Swiss-Prot:O14733

Protein names

MAP2K7, Jnkk2, MAPKK7, MKK7, PRKMK7, SAPKK-4, SAPKK4

Protein function

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

Protein tissue specificity

Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.

Protein sequence and domain

The DVD domain (residues 377-400) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation.; The D domain (residues 37-57) contains a conserved docking site and is required for the binding to MAPK substrates.Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).

Protein cellular localization

Nucleus. Cytoplasm .

Background

c-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activated protein (MAP) kinase family, regulates gene expression in response to various extracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, was phosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNK signaling cascade. MKK7 activity was also stimulated by constitutively active forms of Rac and Cdc42Hs, members of the Rho small GTP-binding protein family (1). endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (but not interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor for the Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposed to heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokine tumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiological pathways (2).

Research area

All research areas>Kinases and Phosphatases>MEK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize MAP2K7 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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