Rabbit polyclonal MAPK9 antibody (A1251)

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Reactivity: Human,Mouse,Rat
Applications: WB,IHC,ICC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal MAPK9 antibody (A1251)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,ICC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 ICC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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MAPK9 antibody,JNK-55 antibody,JNK2 antibody,JNK2A antibody,JNK2ALPHA antibody,JNK2B antibody,JNK2BETA antibody,PRKM9 antibody,SAPK antibody,p54a antibody,p54aSAPK antibody
C Jun kinase 2 antibody|c Jun N terminal kinase 1 antibody|c Jun N terminal kinase 2 antibody|c Jun N terminal kinase 3 antibody|c-Jun N-terminal kinase 1 antibody|JNK 46 antibody|JNK 55 antibody|JNK antibody|JNK-46 antibody|JNK1 antibody|JNK1A2 antibody|JNK2 antibody|JNK21B1/2 antibody|JNK2A antibody|JNK2ALPHA antibody|JNK2B antibody|JNK2BETA antibody|JNK3 alpha protein kinase antibody|JNK3 antibody|JNK3A antibody|Jun kinase antibody|JUN N terminal kinase antibody|MAP kinase 10 antibody|MAP kinase 8 antibody|MAP kinase 9 antibody|MAP kinase p49 3F12 antibody|MAPK 10 antibody|MAPK 8 antibody|MAPK 9 antibody|MAPK10 antibody|mapk8 antibody|MAPK9 antibody|Mitogen activated protein kinase 10 antibody|Mitogen activated protein kinase 8 antibody|Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody|Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody|Mitogen activated protein kinase 9 antibody|Mitogen-activated protein kinase 8 antibody|MK08_HUMAN antibody|p493F12 antibody|p54a antibody|p54aSAPK antibody|p54bSAPK antibody|PRKM10 antibody|PRKM8 antibody|PRKM9 antibody|SAPK antibody|SAPK(beta) antibody|SAPK1 antibody|SAPK1a antibody|SAPK1b antibody|SAPK1c antibody|Stress activated protein kinase 1 antibody|Stress activated protein kinase 1a antibody|Stress activated protein kinase 1b antibody|Stress activated protein kinase 1c antibody|Stress activated protein kinase beta antibody|Stress activated protein kinase JNK1 antibody|Stress activated protein kinase JNK2 antibody|Stress activated protein kinase JNK3 antibody|Stress-activated protein kinase 1c antibody|Stress-activated protein kinase JNK1 antibody|Anti-JNK2 antibody [EP1595Y] (ab76125)

SCBT cat No: sc-7345|sc-1648|

Name

Rabbit polyclonal MAPK9 antibody

Catalogue No.

A1251

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human MAPK9

Host

Rabbit

Applications

WB, IHC, ICC, IF, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
ICC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 48kDa/Observed: Refer to Figures

Formulation

MAPK9 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

MAPK9 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MAPK9 antibody,JNK-55 antibody,JNK2 antibody,JNK2A antibody,JNK2ALPHA antibody,JNK2B antibody,JNK2BETA antibody,PRKM9 antibody,SAPK antibody,p54a antibody,p54aSAPK antibody

Database links

Human UniProt/Swiss-Prot:P45984

Protein names

MAPK9,JNK-55,JNK2,JNK2A,JNK2ALPHA,JNK2B,JNK2BETA,PRKM9,SAPK,p54a,p54aSAPK

Protein function

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock .; MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.

Protein sequence and domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro.

Protein cellular localization

Cytoplasm . Nucleus .

Background

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

Research area

All research areas>Kinases and Phosphatases>JNK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize MAPK9 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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