Rabbit Polyclonal Mcl-1 antibody (STJ96525)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Mcl-1 antibody (STJ96525)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Mcl-1 Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

MCL1 antibody, BCL2L3 antibody,|Myeloid cell leukemia sequence 1 isoform 1 antibody|OTTHUMP00000032794 antibody|OTTHUMP00000032795 antibody|TM antibody|Anti-MCL1 antibody [Y37] (ab32087)
SCBT cat No: sc-12756|sc-53951|sc-74436|sc-377487|sc-74437|sc-20679|sc-69841|sc-69840|sc-69839|sc-69838|sc-56152|sc-53971|sc-819|sc-56427|sc-958|

Name

Mcl-1 Polyclonal Antibody

Catalogue No.

STJ96525

Reactivity

Human, Mouse, Rat

Specificity

Mcl-1 Polyclonal Antibody detects endogenous levels of Mcl-1 protein.

Immunogen

Synthesized peptide derived from Mcl-1 at AA range internal

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

39 kDa

Formulation

Mcl-1 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Mcl-1 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Induced myeloid leukemia cell differentiation protein Mcl-1 antibody, Bcl-2-like protein 3 antibody, Bcl2-L-3 antibody, Bcl-2-related protein EAT/mcl1 antibody, mcl1/EAT antibody

Database links

Human UniProt/Swiss-Prot:Q07820;Mouse UniPort/Swiss-Prot: P97287;Rat UniProt/Swiss-Port: Q9Z1P3;Human Entrez Gene: 4170;Mouse Entrez Gene: 17210;Rat Entrez Gene: Rn.129914

Protein names

Induced myeloid leukemia cell differentiation protein Mcl-1 , Bcl-2-like protein 3 , Bcl2-L-3 , Bcl-2-related protein EAT/mcl1 , mcl1/EAT

Protein function

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Protein tissue specificity

Expression increases early during phorbol ester-induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell line ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat shock-induced differentiation. Expression increases early during retinoic acid-induced differentiation.

Protein sequence and domain

Belongs to the Bcl-2 family.

Protein post-translational modifications

Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. / Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. / Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. / Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.

Protein cellular localization

Membrane / Cytoplasm / Mitochondrion / Nucleus > nucleoplasm

Research area

All research areas>Tumor Suppressor/Apoptosis>Mcl-1
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize Mcl-1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Mouse kidney tissue

1: Mcl-1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: Mcl-1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: Mcl-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung tissue

1: Mcl-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: Mcl-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: Mcl-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: Mcl-1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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