Rabbit polyclonal MCM2 antibody (A1056)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal MCM2 antibody (A1056)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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MCM2 antibody,BM28 antibody,CCNL1 antibody,CDCL1 antibody,D3S3194 antibody,KIAA0030 antibody,MGC10606 antibody,MITOTIN antibody,cdc19 antibody
BM28 antibody|CCNL 1 antibody|CCNL1 antibody|CDC like 1 antibody|cdc19 antibody|CDCL 1 antibody|CDCL1 antibody|Cell devision cycle like 1 antibody|Cyclin like 1 antibody|D3S3194 antibody|DNA replication licensing factor MCM2 antibody|KIAA0030 antibody|MCM 2 antibody|MCM2 antibody|MCM2 minichromosome maintenance deficient 2 mitotin antibody|MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody|MCM2 minichromosome maintenance deficient 2, mitotin antibody|MCM2_HUMAN antibody|MGC10606 antibody|Minichromosome maintenance complex component 2 antibody|Minichromosome maintenance deficient 2 (mitotin) antibody|Minichromosome maintenance deficient 2 mitotin antibody|Minichromosome maintenance protein 2 antibody|Minichromosome maintenance protein 2 homolog antibody|Mitotin antibody|Nuclear protein BM28 antibody|OTTHUMP00000216047 antibody|OTTHUMP00000216050 antibody|Anti-MCM2 antibody (ab4461)

SCBT cat No: sc-56321|sc-73572|sc-373702|sc-10771|sc-9839|sc-56322|

Name

Rabbit polyclonal MCM2 antibody

Catalogue No.

A1056

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human MCM2

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:100 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 102kDa/Observed: Refer to Figures

Formulation

MCM2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

MCM2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MCM2 antibody,BM28 antibody,CCNL1 antibody,CDCL1 antibody,D3S3194 antibody,KIAA0030 antibody,MGC10606 antibody,MITOTIN antibody,cdc19 antibody

Database links

Human UniProt/Swiss-Prot:P49736

Protein names

MCM2,BM28,CCNL1,CDCL1,D3S3194,KIAA0030,MGC10606,MITOTIN,cdc19

Protein function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for ‘once per cell cycle’ DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

Protein sequence and domain

Belongs to the MCM family.; Contains 1 MCM domain.

Protein post-translational modifications

Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.

Protein cellular localization

Nucleus .

Background

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).

Research area

All research areas>Cell Cycle Proteins>MCM
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize MCM2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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