Rabbit polyclonal METTL3 antibody (A8370)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal METTL3 antibody (A8370)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal METTL3 antibody.

Alternative names:

Check alternative names for the antibody

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M6A antibody, IME4 antibody, Spo8 antibody, MT-A70 antibody
adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase antibody|IME4 antibody|M6A antibody|Methyltransferase like protein 3 antibody|Methyltransferase-like protein 3 antibody|METTL3 antibody|mRNA (2′-O-methyladenosine-N(6)-)-methyltransferase antibody|mRNA m(6)A methyltransferase antibody|MT-A70 antibody|MTA70 antibody|MTA70_HUMAN antibody|N6 adenosine methyltransferase 70 kDa subunit antibody|N6-adenosine-methyltransferase 70 kDa subunit antibody|Anti-METTL3 antibody (ab195352)

SCBT cat No: sc-104990|sc-104991|

Name

Rabbit polyclonal METTL3 antibody

Catalogue No.

A8370

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human METTL3

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:100 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 64kDa/Observed: Refer to figures

Formulation

METTL3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

METTL3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

M6A antibody, IME4 antibody, Spo8 antibody, MT-A70 antibody

Database links

Human UniProt/Swiss-Prot:Q86U44

Protein names

M6A, IME4, Spo8, MT-A70

Protein function

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some RNAs and regulates the circadian clock, differentiation of embryonic stem cells and primary miRNA processing . In the heterodimer formed with METTL14, METTL3 probably constitutes the catalytic core . N6-methyladenosine (m6A), which takes place at the 5′-[AG]GAC-3′ consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability . M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop by putting mRNA production on a fast-track for facilitating nuclear processing, thereby providing an early point of control in setting the dynamics of the feedback loop (By similarity). M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs (By similarity). M6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs) . METTL3 also mediates methylation of pri-miRNAs, marking them for recognition and processing by DGCR8 . Acts as a positive regulator of mRNA translation independently of the methyltransferase activity: promotes translation by interacting with the translation initiation machinery in the cytoplasm . Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation .

Protein tissue specificity

Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

Protein sequence and domain

Gate loop 1 and gate loop 2 regions are adjacent to the S-adenosyl-L-homocysteine-binding site and display large conformational changes upon ligand-binding. They may play an important role in adenosine recognition. The interface loop contributes to the heterodimer interaction.Belongs to the MT-A70-like family.

Protein cellular localization

Nucleus . Nucleus speckle . Cytoplasm . Note: Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components.

Background

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Note

AntibodyPlus can customize METTL3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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