Rabbit polyclonal MITF antibody (A1255)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal MITF antibody (A1255)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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MITF antibody,MI antibody,WS2A antibody,bHLHe32 antibody
BHLHE32 antibody|bHLHe32 antibody|Class E basic helix-loop-helix protein 32 antibody|CMM8 antibody|Homolog of mouse microphthalmia antibody|Mi antibody|Microphthalmia associated transcription factor antibody|Microphthalmia, mouse, homolog of antibody|Microphthalmia-associated transcription factor antibody|MITF antibody|MITF_HUMAN antibody|mitfa antibody|nacre antibody|WS2 antibody|WS2A antibody|z3A.1 antibody|Anti-MiTF antibody [C5] – ChIP Grade (ab12039)

SCBT cat No: sc-52938|sc-71588|sc-71587|sc-11002|sc-56725|sc-56726|sc-25386|sc-10999|sc-56433|

Name

Rabbit polyclonal MITF antibody

Catalogue No.

A1255

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human MITF

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 59kDa/Observed: Refer to Figures

Formulation

MITF antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

MITF antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MITF antibody,MI antibody,WS2A antibody,bHLHe32 antibody

Database links

Human UniProt/Swiss-Prot:O75030

Protein names

MITF,MI,WS2A,bHLHe32

Protein function

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5′-CACGTG-3′) found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Protein tissue specificity

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.

Protein sequence and domain

Belongs to the MiT/TFE family.; Contains 1 bHLH (basic helix-loop-helix) domain.

Protein post-translational modifications

Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. Phosphorylated at Ser-180 and Ser-516 following KIT signaling, trigerring a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome.; Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation.

Protein cellular localization

Nucleus.

Background

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Research area

All research areas>Transcription Regulators>MITF
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize MITF antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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