Rabbit polyclonal MLST8 antibody (A1059)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal MLST8 antibody (A1059)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:10 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal MLST8 antibody.

Alternative names:

Check alternative names for the antibody

Expand

GBL antibody,LST8 antibody,MGC111011 antibody,POP3 antibody,WAT1 antibody
G protein beta subunit like antibody|G protein beta subunit-like antibody|Gable antibody|GbetaL antibody|GBL antibody|GBL protein antibody|LST8 antibody|LST8_HUMAN antibody|Mammalian lethal with SEC13 protein 8 antibody|MGC111011 antibody|mLST8 antibody|MTOR associated protein LST8 homolog (S. cerevisiae) antibody|POP3 antibody|POP3 homolog (S. pombe) antibody|Protein GbetaL antibody|Target of rapamycin complex subunit LST8 antibody|TORC subunit LST8 antibody|WAT1 antibody|WAT1 homolog (S. pombe) antibody|Anti-G protein beta subunit like antibody (ab25974)

SCBT cat No: sc-70279|sc-514982|

Name

Rabbit polyclonal MLST8 antibody

Catalogue No.

A1059

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human MLST8

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:10 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 36kDa/Observed: Refer to Figures

Formulation

MLST8 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

MLST8 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

GBL antibody,LST8 antibody,MGC111011 antibody,POP3 antibody,WAT1 antibody

Database links

Human UniProt/Swiss-Prot:Q9BVC4

Protein names

GBL,LST8,MGC111011,POP3,WAT1

Protein function

Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at ‘Thr-389’, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 ‘Ser-473’ phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on ‘Thr-308’ by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at ‘Ser-422’. mTORC2 also modulates the phosphorylation of PRKCA on ‘Ser-657’.

Protein tissue specificity

Broadly expressed, with highest levels in skeletal muscle, heart and kidney.

Protein sequence and domain

Belongs to the WD repeat LST8 family.; Contains 7 WD repeats.

Protein cellular localization

Cytoplasm .

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GbetaL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GbetaL, and rictor (1). GbetaL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GbetaL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).

Research area

All research areas>GDP/GTP Binding Proteins>Heterotrimeric G Protein
(View all antibody categories related to GDP/GTP Binding Proteins)

Note

AntibodyPlus can customize MLST8 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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