Rabbit Polyclonal MMP-9 antibody (STJ96722)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal MMP-9 antibody (STJ96722)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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MMP9 antibody, CLG4B antibody,|82 kDa matrix metalloproteinase-9 antibody|92 kDa gelatinase antibody|92 kDa type IV collagenase antibody|CLG 4B antibody|CLG4B antibody|Collagenase Type 4 beta antibody|Collagenase type IV 92 KD antibody|EC 3.4.24.35 antibody|Gelatinase 92 KD antibody|Gelatinase B antibody|Gelatinase beta antibody|GelatinaseB antibody|GELB antibody|Macrophage gelatinase antibody|MANDP2 antibody|Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody|Matrix Metalloproteinase 9 antibody|MMP 9 antibody|MMP-9 antibody|MMP9 antibody|MMP9_HUMAN antibody|Type V collagenase antibody|Anti-MMP9 antibody (ab38898)
SCBT cat No: sc-21736|sc-107373|sc-163626|sc-130677|sc-373919|sc-373917|sc-398505|sc-398738|

Name

MMP-9 Polyclonal Antibody

Catalogue No.

STJ96722

Reactivity

Human

Specificity

MMP-9 Polyclonal Antibody detects endogenous levels of MMP-9 protein.

Immunogen

Synthesized peptide derived from MMP-9 at AA range INTERNAL

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

78 kDa

Formulation

MMP-9 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

MMP-9 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Matrix metalloproteinase-9 antibody, MMP-9 antibody, 92 kDa gelatinase antibody, 92 kDa type IV collagenase antibody, Gelatinase B antibody, GELB antibody

Database links

Human UniProt/Swiss-Prot:P14780;Mouse UniPort/Swiss-Prot: P41245;Rat UniProt/Swiss-Port: P50282;Human Entrez Gene: 4318;Mouse Entrez Gene: 17395;Rat Entrez Gene: Rn.10209

Protein names

Matrix metalloproteinase-9 , MMP-9 , 92 kDa gelatinase , 92 kDa type IV collagenase , Gelatinase B , GELB

Protein function

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-, -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. / Cleavage of gelatin types I and V and collagen types IV and V. / Zn2+ / Ca2+ / Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1.

Protein tissue specificity

Produced by normal alveolar macrophages and granulocytes.

Involvement in disease

Intervertebral disc disease (IDD) [MIM:603932]: A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. . Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.; Metaphyseal anadysplasia 2 (MANDP2) [MIM:613073]: A bone development disorder characterized by skeletal anomalies that resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. / Belongs to the peptidase M10A family. / Contains 3 fibronectin type-II domains. / Contains 4 hemopexin repeats.

Protein post-translational modifications

Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. / N- and O-glycosylated.

Protein cellular localization

Secreted > extracellular space > extracellular matrix

Research area

All research areas>Synthesis and Degradation>MMP
(View all antibody categories related to Synthesis and Degradation)

Note

AntibodyPlus can customize MMP-9 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver tissue

1: MMP-9 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human kidney tissue

1: MMP-9 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: MMP-9 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: MMP-9 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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