Rabbit polyclonal MSH6 antibody (A0983)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal MSH6 antibody (A0983)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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MSH6 antibody,GTBP antibody,HNPCC5 antibody,HSAP antibody
DNA mismatch repair protein Msh6 antibody|G/T mismatch binding protein antibody|G/T mismatch-binding protein antibody|GTBP antibody|GTMBP antibody|hMSH6 antibody|HNPCC 5 antibody|HNPCC5 antibody|HSAP antibody|MSH 6 antibody|MSH6 antibody|MSH6_HUMAN antibody|mutS (E. coli) homolog 6 antibody|MutS alpha 160 kDa subunit antibody|MutS homolog 6 (E. coli) antibody|mutS homolog 6 antibody|MutS-alpha 160 kDa subunit antibody|p160 antibody|Sperm associated protein antibody|Anti-MSH6 antibody [EPR3945] (ab92471)

SCBT cat No: sc-137015|sc-271979|sc-10798|sc-1243|sc-1242|

Name

Rabbit polyclonal MSH6 antibody

Catalogue No.

A0983

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human MSH6

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 153kDa/Observed: Refer to Figures

Formulation

MSH6 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

MSH6 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MSH6 antibody,GTBP antibody,HNPCC5 antibody,HSAP antibody

Database links

Human UniProt/Swiss-Prot:P52701

Protein names

MSH6,GTBP,HNPCC5,HSAP

Protein function

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP–>ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated ‘Lys-36’ of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Protein sequence and domain

The PWWP domain specifically recognizes and binds trimethylated ‘Lys-36’ of histone H3 (H3K36me3).Belongs to the DNA mismatch repair MutS family.; Contains 1 PWWP domain.

Protein post-translational modifications

The N-terminus is blocked.; Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

Protein cellular localization

Nucleus . Chromosome . Note: Associates with H3K36me3 via its PWWP domain.

Background

The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-alpha dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-alpha is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4).

Research area

All research areas>Tumor Suppressor/Apoptosis>GTBP
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize MSH6 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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