Rabbit polyclonal MYOC antibody (A1589)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal MYOC antibody (A1589)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

GPOA antibody, JOAG antibody, TIGR antibody, GLC1A antibody, JOAG1 antibody, myocilin antibody
GLC1A antibody|GPOA antibody|JOAG antibody|JOAG1 antibody|Mutated trabecular meshwork-induced glucocorticoid response protein antibody|MYOC antibody|MYOC_HUMAN antibody|Myocilin antibody|Myocilin, trabecular meshwork inducible glucocorticoid antibody|TIGR antibody|Trabecular meshwork induced glucocorticoid response protein antibody|Trabecular meshwork-induced glucocorticoid response protein antibody|Anti-Myocilin antibody (ab41552)

SCBT cat No: sc-515500|sc-21245|sc-137233|sc-20976|sc-21243|

Name

Rabbit polyclonal MYOC antibody

Catalogue No.

A1589

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human MYOC

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 57kDa/Observed: Refer to Figures

Formulation

MYOC antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

MYOC antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

GPOA antibody, JOAG antibody, TIGR antibody, GLC1A antibody, JOAG1 antibody, myocilin antibody

Database links

Human UniProt/Swiss-Prot:Q99972

Protein names

GPOA, JOAG, TIGR, GLC1A, JOAG1, myocilin

Protein function

Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork.

Protein tissue specificity

Detected in aqueous humor . Detected in the eye (at protein level) . Widely expressed. Highly expressed in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart, and bone marrow-derived mesenchymal stem cells. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and seems to be expressed at higher levels than in normal eyes, regardless of the type or clinical severity of glaucoma. The myocilin 35 kDa fragment is detected in aqueous humor and at to a lesser extend in iris and ciliary body.

Protein sequence and domain

Contains 1 olfactomedin-like domain.

Protein post-translational modifications

Different isoforms may arise by post-translational modifications.; Glycosylated.; Palmitoylated.; Undergoes a calcium-dependent proteolytic cleavage at Arg-226 by CAPN2 in the endoplasmic reticulum. The result is the production of two fragments, one of 35 kDa containing the C-terminal olfactomedin-like domain, and another of 20 kDa containing the N-terminal leucine zipper-like domain.

Protein cellular localization

Secreted . Golgi apparatus . Cytoplasmic vesicle . Secreted, extracellular space. Secreted, extracellular space, extracellular matrix . Secreted, exosome . Mitochondrion . Mitochondrion intermembrane space . Mitochondrion inner membrane . Mitochondrion outer membrane . Rough endoplasmic reticulum . Cell projection. Cell projection, cilium . Note: Located preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells, and in the rough endoplasmic reticulum . It is only imported to mitochondria in the trabecular meshwork . Localizes to the Golgi apparatus in Schlemm’s canal endothelial cells . Appears in the extracellular space of trabecular meshwork cells by an unconventional mechanism, likely associated with exosome-like vesicles . Localizes in trabecular meshwork extracellular matrix .; Myocilin, C-terminal fragment: Secreted.; Myocilin, N-terminal fragment: Endoplasmic reticulum. Note: Remains retained in the endoplasmic reticulum.

Background

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYOC has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989).

Note

AntibodyPlus can customize MYOC antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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