Rabbit polyclonal NCBP2 antibody (A7293)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NCBP2 antibody (A7293)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal NCBP2 antibody.

Alternative names:

Check alternative names for the antibody

Expand

CBC2 antibody, NIP1 antibody, CBP20 antibody, PIG55 antibody
20 kDa nuclear cap binding protein antibody|CBC2 antibody|CBP20 antibody|Cell proliferation inducing gene 55 protein antibody|NCBP 2 antibody|NCBP 20 kDa subunit antibody|NCBP interacting protein 1 antibody|NIP1 antibody|Nuclear cap binding protein subunit 2 antibody|PIG55 antibody|Anti-NCBP2 antibody (ab91560)

SCBT cat No: sc-137123|sc-48793|

Name

Rabbit polyclonal NCBP2 antibody

Catalogue No.

A7293

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human NCBP2

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 18kDa/Observed: Refer to figures

Formulation

NCBP2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NCBP2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CBC2 antibody, NIP1 antibody, CBP20 antibody, PIG55 antibody

Database links

Human UniProt/Swiss-Prot:P52298

Protein names

CBC2, NIP1, CBP20, PIG55

Protein function

Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5′ cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5′ end of mRNA and to mRNA export in a 5′ to 3′ direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in ‘failsafe’ NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus .

Protein sequence and domain

Belongs to the RRM NCBP2 family.; Contains 1 RRM (RNA recognition motif) domain.

Protein cellular localization

Nucleus . Cytoplasm .

Background

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5′ cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3′-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene.

Research area

All research areas>Transcription Regulators>CBP20
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize NCBP2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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